Development of Polymerase Chain Reaction assays with host-specific internal controls for Chlamydophila abortus

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dc.contributor.authorCantekin, Z.
dc.contributor.authorSolmaz, H.
dc.contributor.authorErgun, Y.
dc.contributor.authorOzmen, M.
dc.date.accessioned2024-09-18T20:08:21Z
dc.date.available2024-09-18T20:08:21Z
dc.date.issued2015
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractChlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell culture or embryonated eggs for growth. Therefore, culture-independent detection methods such as the polymerase chain reaction are increasingly important and needed. The aim of this study was to develop a polymerase chain reaction assay with an internal control for detection of C. abortus in clinical samples. Using newly-designed two primer sets specific for C. abortus, the polymerase chain reaction assay was first tested with positive and negative control DNA and its sensitivity and specificity were determined. A new polymerase chain reaction protocol was developed by combining the new primer pair sets with bovine (12SM-FW and 12SBT-REV2) and ruminant host-specific primer sets (12S-FW and 12S-REV). In conclusion, the developed polymerase chain reaction assays can potentially be used for direct detection of Chlamydophila abortus in bovine and ruminant samples.en_US
dc.identifier.doi10.17221/7919-VETMED
dc.identifier.endpageU7en_US
dc.identifier.issn0375-8427
dc.identifier.issn1805-9392
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84923034133en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.17221/7919-VETMED
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8786
dc.identifier.volume60en_US
dc.identifier.wosWOS:000349028900001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherCzech Academy Agricultural Sciencesen_US
dc.relation.ispartofVeterinarni Medicinaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChlamydophila abortusen_US
dc.subjectinternal controlen_US
dc.subjectPCRen_US
dc.titleDevelopment of Polymerase Chain Reaction assays with host-specific internal controls for Chlamydophila abortusen_US
dc.typeArticleen_US

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