Development of a BoHV-4 viral vector expressing tgD of BoHV-1 and evaluation of its immunogenicity in mouse model

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dc.authoridAlkan, Feray/0000-0003-3854-6503
dc.authoridAkkutay-Yoldar, Zeynep/0000-0002-1178-5347
dc.authoridOzkul, Aykut/0000-0001-5008-9443
dc.contributor.authorBilge-Dagalp, Seval
dc.contributor.authorFarzani, Touraj Aligholipour
dc.contributor.authorDogan, Firat
dc.contributor.authorYoldar, Zeynep Akkutay
dc.contributor.authorOzkul, Aykut
dc.contributor.authorAlkan, Feray
dc.contributor.authorDonofrio, Gaetano
dc.date.accessioned2024-09-18T20:04:44Z
dc.date.available2024-09-18T20:04:44Z
dc.date.issued2021
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn recent years, Bovine herpesvirus 4 (BoHV-4) has emerged as an attractive gene delivery viral vector, mainly for vaccination purposes in the veterinary field. In the present study, a new infectious clone of the BoHV-4 genome carrying a bacterial artificial chromosome vector (BoHV-4-BAC) was developed by homologous recombination in mammalian cell culture and bacterial systems, and exploited to express a truncated form of glycoprotein D (tgD) of Bovine herpesvirus 1 (BoHV-1) (BoHV-4-tgD increment TK) as a vaccine candidate. This construct's immunogenicity was compared to a DNA vector expressing the same antigen (pC-tgD) in a BALB/c mouse model. After the mice were immunized, total and specific antibody responses, cytokine responses, total splenocyte cells proliferation/cytotoxicity, and virus neutralization assays were conducted to analyze the immune response elicited by both constructs. Mice from both vaccine groups developed significant humoral and cellular immune responses after a booster dose regime was conducted on day 28 post-injection. In almost all immunological assays, BoHV-4-tgD Delta TK induced as high an immune response as pC-tgD. In both vaccine constructs, neutralizing antibodies were a significant determining factor in protection against BoHV-1, even after the first injection. We conclude that a BoHV-4-based viral vector offers an effective immunization strategy as an alternative to DNA-based immunization platforms, at least to combat BoHV-1.en_US
dc.description.sponsorshipScientific Research Projects, Ankara University (BAP) [17B0239004]en_US
dc.description.sponsorshipThis study was supported by a grant from Scientific Research Projects, Ankara University (BAP; Project number: 17B0239004).en_US
dc.identifier.doi10.1007/s42770-021-00525-z
dc.identifier.endpage1133en_US
dc.identifier.issn1517-8382
dc.identifier.issn1678-4405
dc.identifier.issue3en_US
dc.identifier.pmid34255309en_US
dc.identifier.scopus2-s2.0-85110688289en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1119en_US
dc.identifier.urihttps://doi.org/10.1007/s42770-021-00525-z
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8332
dc.identifier.volume52en_US
dc.identifier.wosWOS:000673021700002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBrazilian Journal of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBovine herpesvirus 4en_US
dc.subjectViral vectoren_US
dc.subjectBovine herpesvirus 1en_US
dc.subjectTgDen_US
dc.subjectImmune responseen_US
dc.titleDevelopment of a BoHV-4 viral vector expressing tgD of BoHV-1 and evaluation of its immunogenicity in mouse modelen_US
dc.typeArticleen_US

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