COMPARISON OF N-ACETYL-L-CYSTEINE AND L-CYSTEINE IN RESPECT TO THEIR TRANSMEMBRANE FLUXES
dc.contributor.author | Yildiz, D. | |
dc.contributor.author | Arik, M. | |
dc.contributor.author | Cakir, Y. | |
dc.contributor.author | Civi, Z. | |
dc.date.accessioned | 2024-09-18T20:14:59Z | |
dc.date.available | 2024-09-18T20:14:59Z | |
dc.date.issued | 2009 | |
dc.department | Hatay Mustafa Kemal Üniversitesi | en_US |
dc.description.abstract | The objective of the present study was to compare cysteine and N-acetyl-L-cysteine in respect to their transmembrane fluxes and find out which one is a better available precursor for the cells and thus better supports the intracellular glutathione synthesis. Cysteine can directly participate in glutathione synthesis, whereas N-acetyl-L-cysteine must be first deacetylated before its incorporation to glutathione. In the present study we investigated and compared the efficiencies of cysteine and N-acetyl-L-cysteine influx and efflux through the erythrocyte membrane. Erythrocytes transported both cysteine and N-acetyl-L-cysteine in a concentration-dependent manner. However, our results demonstrated that cysteine crosses the erythrocyte membranes more efficiently than N-acetyl-L-cysteine does. Treatment of erythrocytes with 5 mM of cysteine or N-acetyl-L-cysteine for 1 hr raised the intracellular free sulfhydryl group (free-SH) levels to 3.37 +/- 0.006 or 2.23 +/- 0.08 mu mol/ml erythrocyte, respectively. Cysteine more effectively than N-acetyl-L-cysteine restored the intracellular free-SH level lowered beforehand. In erythrocytes previously depleted of free-SH, 5 mM cysteine raised the free-SH level to 1.45 +/- 0.075 mu mole/ml within I hr, whereas N-acetyl-L-cysteine at the same concentration raised this level to 0.377 +/- 0.034 mu mole/ml only. The results of our study also revealed that both cysteine and N-acetyl-L-cysteine influx and efflux processes are temperature dependent, indicating that their transport requires biological activity. Our results demonstrate that cysteine is a better thiol precursor for the erythrocytes. Availability of cysteine for the cells is higher than that of N-acetyl-L-cysteine. | en_US |
dc.identifier.endpage | 148 | en_US |
dc.identifier.issn | 0233-4755 | |
dc.identifier.issue | 2 | en_US |
dc.identifier.startpage | 143 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12483/9352 | |
dc.identifier.volume | 26 | en_US |
dc.identifier.wos | WOS:000264905900007 | en_US |
dc.identifier.wosquality | Q4 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.language.iso | en | en_US |
dc.publisher | Mezhdunarodnaya Kniga | en_US |
dc.relation.ispartof | Biologicheskie Membrany | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Cysteine | en_US |
dc.subject | N-acetyl-L-cysteine (NAC) | en_US |
dc.subject | erythrocytes | en_US |
dc.subject | transmembrane transport | en_US |
dc.title | COMPARISON OF N-ACETYL-L-CYSTEINE AND L-CYSTEINE IN RESPECT TO THEIR TRANSMEMBRANE FLUXES | en_US |
dc.type | Article | en_US |