Geobacillus thermodenitrificans subsp calidus, subsp nov., a thermophilic and ?-glucosidase producing bacterium isolated from Kizilcahamam, Turkey

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dc.authoridColeri Cihan, Arzu/0000-0002-7289-6251
dc.contributor.authorCihan, Arzu Coleri
dc.contributor.authorOzcan, Birgul
dc.contributor.authorTekin, Nilgun
dc.contributor.authorCokmus, Cumhur
dc.date.accessioned2024-09-18T20:15:17Z
dc.date.available2024-09-18T20:15:17Z
dc.date.issued2011
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAn alpha-glucosidase producing, thermophilic, facultatively anaerobic, and endospore-forming, motile, rod-shaped bacterial strain F84b(T) was isolated from a high temperature well-pipeline sediment sample in Kizilcahamam, Turkey. The growth occurred at temperatures, pH and salinities ranging from 45 to 69 degrees C (optimum 60 degrees C), 7.0 to 8.5 (optimum 8.0) and 0 to 5% (w/v) (optimum 3.5%), respectively. Strain F84b(T) was able to grow on a wide range of carbon sources. Starch and tyrosine utilization, amylase, catalase and oxidase activities, nitrate reduction, and gas production from nitrate were all positive. The G+C content of the genomic DNA was 49.6 mol%. The menaquinone content was MK-7. The dominant cellular fatty acids were iso-C17:0, iso-C15:0, and C16:0. In phylogenetic analysis of 16S rRNA gene sequence, strain F84b(T) showed high sequence similarity to Geobacillus thermodenitrificans (99.8%) and to Geobacillus subterraneus (99.3%) with DNA hybridization values of 74.3% and 29.1%, respectively. In addition, the Rep-PCR and the intergenic 16S-23S rRNA gene fingerprinting profiles differentiated strain F84b(T) from the Geobacillus species studied. The results obtained from the physiological and biochemical characters, the menaquinone contents, the borderline DNA-DNA hybridization homology, and the genomic fingerprinting patterns had allowed phenotypic, chemotaxonomic and genotypic differentiation of strain F84b(T) from G. thermodenitrificans. Therefore, strain F84b(T) is assigned to be a new subspecies of G. thermodenitrificans, for which the name Geobacillus thermodenitrificans subsp. calidus, subsp. nov. is proposed (The type strain F84b(T) = DSM 22629(T) = NCIMB 14582(T)).en_US
dc.description.sponsorshipFundamental Research Group of The Scientific and Technological Research Council of Turkey (TUBITAK) [TBAG-HD-320(107T286), TBAG-HD126 (105T039)]en_US
dc.description.sponsorshipThis study was supported by grants from The Fundamental Research Group of The Scientific and Technological Research Council of Turkey (TUBITAK) (project numbers of TBAG-HD-320(107T286) and TBAG-HD126 (105T039)). We thank Dr. Mehlika Benli, Biology Department, Ankara University, Ankara, Turkey for performing the electron microscopy. In addition we are very grateful to Prof. Hans G. Truper, University of Bonn, Germany for proposing and correcting the nomenclature of the new subspecies.en_US
dc.identifier.doi10.2323/jgam.57.83
dc.identifier.endpage92en_US
dc.identifier.issn0022-1260
dc.identifier.issn1349-8037
dc.identifier.issue2en_US
dc.identifier.pmid21606609en_US
dc.identifier.scopus2-s2.0-79957877578en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage83en_US
dc.identifier.urihttps://doi.org/10.2323/jgam.57.83
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9551
dc.identifier.volume57en_US
dc.identifier.wosWOS:000296949100002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMicrobiol Res Foundationen_US
dc.relation.ispartofJournal of General and Applied Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDNA-DNA hybridizationen_US
dc.subjectgenomic fingerprintingen_US
dc.subjectGeobacillusen_US
dc.subjectlipiden_US
dc.subject16S rRNA geneen_US
dc.subjecttaxonomyen_US
dc.subjectthermostable alpha-glucosidaseen_US
dc.titleGeobacillus thermodenitrificans subsp calidus, subsp nov., a thermophilic and ?-glucosidase producing bacterium isolated from Kizilcahamam, Turkeyen_US
dc.typeArticleen_US

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