Gelling agents and culture vessels affect in vitro multiplication of banana plantlets

Basit Öğe Kaydı
dc.contributor.authorKacar, Y. A.
dc.contributor.authorBicen, B.
dc.contributor.authorVarol, I.
dc.contributor.authorMendi, Y. Y.
dc.contributor.authorSerce, S.
dc.contributor.authorCetiner, S.
dc.date.accessioned2024-09-18T20:16:35Z
dc.date.available2024-09-18T20:16:35Z
dc.date.issued2010
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAgar is the most commonly used gelling agent in media for plant tissue culture. Because of the high price of tissue-culture-grade agar, attempts have been made to identify suitable alternatives. The type of culture vessel and lid also affects the gaseous composition inside the vessel as well as light penetration. In turn, the vessel affects growth parameters, such as shoot elongation, proliferation and fresh weight, as well as hyper-hydric degradation processes. We examined the effects of different culture vessels, including commercial glass jars, magenta boxes, and disposable containers, as well as different gelling agents (agar-agar, Agargel, Phytagel, and plant agar) on the micropropagation of Dwarf Cavendish bananas in an effort to find a combination that yields large numbers of high-quality seedlings. The different culture vessels did not significantly affect seedling culture success. The medium significantly affected shoot weight. Phytagel resulted in the highest shoot weight (overall mean = 2.4 g), while agar, Agargel and plant agar resulted in 1.7, 2.2 and 2.2 g, respectively. Disposable container/Phytagel and Magenta/Agargel combinations yielded the highest shoot weights (2.9 and 3.0 g, respectively). Mean shoot length increased progressively with subculture (four subcultures were made). The highest mean shoot length was obtained with Phytagel and Agargel media (6.4 and 6.3 cm, respectively). Shoot number was significantly affected by medium only at subculture 4. Overall, the highest mean shoot length was obtained with the Magenta/Agargel combination (8.5 cm). Phytagel and plant agar gave higher mean shoot number than agar and Agargel (2.1, 2.1 and 1.7 and 1.9, respectively). The costs of the media and of the culture vessels need to be taken into account for final choice of the banana shoot culture system.en_US
dc.identifier.doi10.4238/vol9-1gmr744
dc.identifier.endpage424en_US
dc.identifier.issn1676-5680
dc.identifier.issue1en_US
dc.identifier.pmid20309826en_US
dc.identifier.scopus2-s2.0-77952537215en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage416en_US
dc.identifier.urihttps://doi.org/10.4238/vol9-1gmr744
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9610
dc.identifier.volume9en_US
dc.identifier.wosWOS:000277326200035en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherFunpec-Editoraen_US
dc.relation.ispartofGenetics and Molecular Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDwarf Cavendishen_US
dc.subjectMagenta vesselen_US
dc.subjectAgar-agaren_US
dc.subjectAgargelen_US
dc.subjectPhytagelen_US
dc.subjectPlant agaren_US
dc.titleGelling agents and culture vessels affect in vitro multiplication of banana plantletsen_US
dc.typeArticleen_US

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