Determination of antagonistic potential of endophytic bacteria isolated from lettuce against lettuce white mould disease caused by Sclerotinia sclerotiorum
Loading...
Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Lithuanian Research Centre Agriculture & Forestry
Access Rights
info:eu-repo/semantics/openAccess
Abstract
In this study, biocontrol efficiency of endophytic bacterial isolates obtained from the leaf lettuce (Lactuca sativa L.) healthy plants was investigated against white mould disease agent Sclerotinia sclerotiorum in vitro and in vivo conditions. Antagonistic efficiency of endophytic bacterial isolates was determined to inhibit mycelial growth and sclerotial germination, suppress disease incidence caused by S. sclerotiorum. A total of 48 endophytic bacterial isolates were isolated from different tissues of lettuce healthy plants. Mycelial growth and germination of sclerotia of S. sclerotiorum in vitro inhibited 18 bacterial isolates. Bacillus subtilis and B. amyloliquefaciens isolates were found to be the most efficient ones, which significantly inhibited the mycelial growth by 68.1-83.1%, germination of sclerotia by 82.7-89.6% and suppressed disease incidence by 55.7-75% caused by S. sclerotiorum. In addition, the fungal mycelium close to the inhibition zone in dual culture was denser and darker in colour. B. subtilis and B. amyloliquefaciens isolates caused significant morphological alterations in hyphae such as hyphal shrivelling and perforation close to the inhibition zone in dual culture. Significant suppression in the mycelial growth, sclerotial germination and disease incidence caused by endophytic bacterial isolates indicate that isolates of B. subtilis and B. amyloliquefaciens could be considered as possible biocontrol agents against soil-borne fungal diseases.
Description
Keywords
Lactuca sativa, antagonist, biological control, endophytes, Sclerotinia sclerotiorum
Journal or Series
Zemdirbyste-Agriculture
WoS Q Value
Q3
Scopus Q Value
Q3
Volume
108
Issue
4
