Silencing of PrPC (prion protein) expression does not affect Brucella melitensis infection in human derived microglia cells

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dc.contributor.authorErdogan, Suat
dc.contributor.authorDuzguner, Vesile
dc.contributor.authorKucukgul, Altug
dc.contributor.authorAslantas, Ozkan
dc.date.accessioned2024-09-18T20:06:15Z
dc.date.available2024-09-18T20:06:15Z
dc.date.issued2013
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractCellular prion proteins (PrPC) are mainly expressed in the central nervous system where they have antioxidant effects and a role in the endocytosis of bacteria within cells. These proteins also have some crucial biological functions including roles in neurotransmission, signal transduction and programmed cell death. However, the role of prion proteins in neuronal Brucella infection, specifically in the interaction of the pathogen and the host cell is controversial. In the present study, the silencing of PrPC mRNA by small interfering RNA (siRNA) transfection was investigated in human microglia cells infected with Brucella melitensis. More than 70% of prion proteins were down-regulated in microglia by siRNA transfection and this caused a slight decrease in the cellular viability of the control cells. Silencing of PrPC suppressed the antioxidant systems, though it led to an up-regulation of pro-inflammatory cytokines such as IL-12 and TNF-alpha as demonstrated by qRT-PCR analysis. B. melitensis infection of prion protein-silenced cells led to increase host viability, but had no effect on bacterial phagocytosis. According to the present study, there is no significant effect of prion proteins on phagocytosis and intracellular killing of B. melitensis in microglia cells. (c) 2013 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [108O313]en_US
dc.description.sponsorshipThis research was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) (Project no: 108O313). The authors would like to thank Dr. Sandra Spence (Glasgow, UK) for her careful reading and comments that help improve the manuscript.en_US
dc.identifier.doi10.1016/j.rvsc.2013.06.007
dc.identifier.endpage373en_US
dc.identifier.issn0034-5288
dc.identifier.issn1532-2661
dc.identifier.issue2en_US
dc.identifier.pmid23820446en_US
dc.identifier.scopus2-s2.0-84881552479en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage368en_US
dc.identifier.urihttps://doi.org/10.1016/j.rvsc.2013.06.007
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8417
dc.identifier.volume95en_US
dc.identifier.wosWOS:000323861000012en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofResearch in Veterinary Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBrucellaen_US
dc.subjectInflammationen_US
dc.subjectAntioxidant systemen_US
dc.subjectMicrogliaen_US
dc.subjectPrion proteinen_US
dc.subjectsiRNAen_US
dc.titleSilencing of PrPC (prion protein) expression does not affect Brucella melitensis infection in human derived microglia cellsen_US
dc.typeArticleen_US

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