A new disease of strawberry, bacterial blight caused by Erwinia amylovora in Turkey

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dc.authoridSOYLU, Soner/0000-0003-1002-8958
dc.authoridozturk, murat/0000-0002-9677-3651
dc.contributor.authorOzturk, Murat
dc.contributor.authorSoylu, Soner
dc.date.accessioned2024-09-18T20:59:08Z
dc.date.available2024-09-18T20:59:08Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractStrawberry (Fragaria x ananassa Duch.) is one of the high-economic soft fruit crops, which plays an important role in farmers' incomes in Turkey. In a disease survey conducted in 2018 and 2019 growing seasons, typical disease symptoms resembling fire blight caused by Erwinia amylovora were observed in Yozgat and Mersin province of Turkey. The diseased strawberry plants showed intense black to brown necrotic areas in the midribs and veins on the leaf area and the attached stems. Twelve strains of E. amylovora were isolated from naturally infected strawberry plants on the basis of disease symptoms. The strains were initially characterized based on physiological, biochemical characters, and pathogenicity tests and identified using MALDI TOF MS analyses. All strains were identified as E. amylovora based on their protein fingerprint patterns obtained by the MALDI TOF MS system. The identification of representative strains was further confirmed by PCR amplification of a specific region by using taxon-specific primers, sequencing 16S rDNA and 3 housekeeping genes (gapA, mdh and recA). Obtained partial nucleotide sequences of each gene were submitted to BLAST analysis and strains shared 100% (for gapA), 99.45% (for mdh) and 100% (for recA) nucleotide identity with each other and sequence of complete genomes of E. amylovora strains available in GenBank. Constructed phylogenetic trees with concatenated sequences of gapA, mdh and recA genes allowed distinctly to separate strawberry E. amylovora strains from other Erwinia species. Representative bacterial strains were further tested for copper and streptomycin sensitivity. None of the strains grown on the nutrient medium containing 2.56 mM copper sulphate and 5 ppm streptomycin sulphate indicated that strawberry isolates are sensitive to copper and streptomycin for now. This study is the first report of a natural outbreak of bacterial fire blight disease caused by E. amylovora on strawberries in Turkey. Since disease is transmitted by bees, the presence of the pathogen may seriously influence local strawberry production in the future.en_US
dc.description.sponsorshipOffice of Scientific Research Projects Coordination of Yozgat Bozok University [6602c-ZF/18-190]en_US
dc.description.sponsorshipThis study was supported financially by a grant from the Office of Scientific Research Projects Coordination of Yozgat Bozok University (Project no. 6602c-ZF/18-190). The author would like to sincerely thank A. Demirci diligent proofreading of this manuscript. The authors would like to thank Prof. Dr. Aleksa Obradovic for supplying the refence strains of this work.en_US
dc.identifier.doi10.1007/s42161-021-00994-z
dc.identifier.endpage280en_US
dc.identifier.issn1125-4653
dc.identifier.issn2239-7264
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85119040318en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage269en_US
dc.identifier.urihttps://doi.org/10.1007/s42161-021-00994-z
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12396
dc.identifier.volume104en_US
dc.identifier.wosWOS:000719268400003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Plant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectStrawberryen_US
dc.subjectErwinia amylovoraen_US
dc.subjectIdentificationen_US
dc.subjectMLSTen_US
dc.titleA new disease of strawberry, bacterial blight caused by Erwinia amylovora in Turkeyen_US
dc.typeArticleen_US

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