Impact of Enoxaparin plus a Lipoic acid combination on oxidative stress, follicle development and apoptotic damage in ovarian ischemia reperfusion model
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Dosyalar
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Verduci Publisher
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
OBJECTIVE: With this study we aimed at investigating the impact of enoxaparin (E) and alpha-lipoic acid (alpha-LA) on ischemia/reper-fusion (I/R) damage in an experimental ovarian torsion (T) detorsion (D) model. MATERIALS AND METHODS: The study com-prised 80 female albino rats aged 12 weeks. They were divided into 8 groups as follows, with 10 rats in each group: Group 1 (Sham), Group 2 (Ischemia, 3 hours), Group 3 (I/R, 6 hours reper-fusion), Group 4 (I/R, 3 days reperfusion), Group 5 (I/R, E, 6 hours), Group 6 (I/R, E, 3 days), Group 7 (I/R, E+alpha-LA, 6 hours), and Group 8 (I/R, E+alpha-LA, 3 days). Immediately after detorsion, enoxaparin (0.5 mg/kg/day, sc) or alpha-LA (100 mg/kg/day, ip) were administered. Ovarian levels of malondial-dehyde (MDA), glutathione peroxidase (GPx), and catalase were measured. In addition to total An-tioxidant Status (TAS) and Total Oxidative Stress (TOS), the Oxidative Stress Index (OSI) was calcu-lated. Serum levels of AMH, FSH, LH, E2, and pro-gesterone were measured. Ovarian samples were histopathologically examined and they underwent Tag staining for apoptosis.RESULTS: Compared with the sham group, there was a significant increase in TOS, OSI, and MDA levels in I/R group (p<0.001). Compared to Group 2, a significant increase was determined in TOS and OSI in Group 3 (p<0.05). In Group 4, a significant increase was found compared to Group 2 (p<0.01). The lowest level of I/R dam-age and the highest level of antioxidants were found in Group 2, 7, and 8. The highest values of TOS, OSI, and MDA were found in Group 3, Group 4, Group 5, and Group 6 (p<0.001). When the sham group and Group 4 were compared, it was found that AMH and E2 levels decreased (p<0.001), while FSH levels increased (p<0.001). In Group 6, AMH and E2 values were signifi-cantly decreased compared to the sham group (p<0.001). In the histopathological examination, the findings were observed to be parallel to those of the biochemical markers.CONCLUSIONS: Combined use of alpha-Lipoic ac-id and enoxaparin prevents oxidative stress and ovarian damage due to ischemia-reperfusion in-jury better than the use of drugs alone.
Açıklama
Anahtar Kelimeler
a-Lipoic, Enoxaparin, Ovarian torsion, Ischemia, eperfusion, Oxidative stress, Apoptosis
Kaynak
European Review For Medical and Pharmacological Sciences
WoS Q DeÄŸeri
Q2
Scopus Q DeÄŸeri
Cilt
26
Sayı
18
