DETECTION OF FIG MOSAIC VIRUS IN VIRULIFEROUS ERIOPHYID MITE ACERIA FICUS

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dc.authoridMedina, Vicente/0000-0002-1030-2235
dc.authoridElci, Eminur/0000-0002-6434-6321
dc.contributor.authorCaglayan, K.
dc.contributor.authorElci, E.
dc.contributor.authorSerce, C. Ulubas
dc.contributor.authorKaya, K.
dc.contributor.authorGazel, M.
dc.contributor.authorMedina, V.
dc.date.accessioned2024-09-18T20:20:09Z
dc.date.available2024-09-18T20:20:09Z
dc.date.issued2012
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractFig leaves showing typical fig mosaic symptoms on cv. Bursa siyahi (donor plant) were cut under a stereo microscope into small pieces each hosting 10 putatively viruliferous eriophyid mites (Aceria ficus Cotte) and placed directly on the top leaves of healthy Cucumis sativus, Chenopodium quinoa. C. amaranticolor, Nicotiana occidentalis, Catharanthus roseus, Fraxinus excelsior plants, and fig seedlings. Donor and test plants were analyzed by electron microscopy, RT-PCR and sequencing, whereas the mites (ErMs) underwent molecular assays using Fig mosaic virus (FMV)-specific primers. Mite-infested leaves of fig seedlings and C. roseus showed small yellowish spots after 10 days and 6 weeks, respectively, whereas no symptoms were observed in other test or control plants for three months. Electron microscopy observations showed the occurrence of double membrane bodies (DMBs) in the palisade cells of donor and mite-inoculated fig plants, but not in C. roseus. However, 302 bp RT-PCR products specific to FMV were amplified from donor and inoculated figs, C. roseus and ErMs. Nucleotide identity with the sequence of the FMV isolate in GenBank (accession No. AM941711.6) was 87%, 89% and 87% for donor plant (JQ708183), inoculated fig seedlings (JQ708184) and C. roseus (JQ408437, JQ408438), respectively. The sequences obtained from ErMs (JQ408432, JQ408436) showed 87% and 88% nucleotide identity with the reference FMV isolate, respectively. When dsRNA extracts were analyzed to confirm virus presence in inoculated periwinkles, a complex dsRNA profile was obtained, suggestive of infection by a multipartite virus or by multiple viruses. Sequence from RT-PCR amplicons of dsRNA (JX040436) showed 88% identity with those the reference FMV isolate (AM941716.1) and the donor plant (JQ708183). According to these results, Madagascar periwinkle (C. roseus) can be retained as a new experimental host for FMV and A. ficus appears to be able to transmit FMV from fig to periwinkle plants.en_US
dc.description.sponsorshipTUBITAK, Turkey [TOVAG-106O134]en_US
dc.description.sponsorshipThis study was supported by TUBITAK (TOVAG-106O134), Turkey.en_US
dc.identifier.endpage634en_US
dc.identifier.issn1125-4653
dc.identifier.issn2239-7264
dc.identifier.issue3en_US
dc.identifier.startpage629en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10074
dc.identifier.volume94en_US
dc.identifier.wosWOS:000312039500017en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Plant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFig mosaic diseaseen_US
dc.subjectAceria ficusen_US
dc.subjectherbaceous and woody indicatorsen_US
dc.subjectelectron microscopyen_US
dc.subjectRT-PCRen_US
dc.subjectsequencingen_US
dc.titleDETECTION OF FIG MOSAIC VIRUS IN VIRULIFEROUS ERIOPHYID MITE ACERIA FICUSen_US
dc.typeArticleen_US

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