DNA microarrays for identifying fishes

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dc.authoridTINTI, FAUSTO/0000-0002-8649-5387
dc.authoridLandi, Monica/0000-0003-2180-5062
dc.authoridNolte, Manfred/0000-0002-8029-4162
dc.authoridMarteinsson, Viggo/0000-0001-8340-821X
dc.authoridKochzius, Marc/0000-0001-8953-4719
dc.authoridAntoniou, Aglaia (Cilia)/0000-0003-1119-9820
dc.authoridHreggvidsson, Gudmundur Oli/0000-0002-4958-1673
dc.contributor.authorKochzius, M.
dc.contributor.authorNoelte, M.
dc.contributor.authorWeber, H.
dc.contributor.authorSilkenbeumer, N.
dc.contributor.authorHjoerleifsdottir, S.
dc.contributor.authorHreggvidsson, G. O.
dc.contributor.authorMarteinsson, V.
dc.date.accessioned2024-09-18T20:15:17Z
dc.date.available2024-09-18T20:15:17Z
dc.date.issued2008
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn many cases marine organisms and especially their diverse developmental stages are difficult to identify by morphological characters. DNA-based identification methods offer an analytically powerful addition or even an alternative. In this study, a DNA microarray has been developed to be able to investigate its potential as a tool for the identification of fish species from European seas based on mitochondrial 16S rDNA sequences. Eleven commercially important fish species were selected for a first prototype. Oligonucleotide probes were designed based on the 16S rDNA sequences obtained from 230 individuals of 27 fish species. In addition, more than 1200 sequences of 380 species served as sequence background against which the specificity of the probes was tested in silico. Single target hybridisations with Cy5-labelled, PCR-amplified 16S rDNA fragments from each of the 11 species on microarrays containing the complete set of probes confirmed their suitability. True-positive, fluorescence signals obtained were at least one order of magnitude stronger than false-positive cross-hybridisations. Single nontarget hybridisations resulted in cross-hybridisation signals at approximately 27% of the cases tested, but all of them were at least one order of magnitude lower than true-positive signals. This study demonstrates that the 16S rDNA gene is suitable for designing oligonucleotide probes, which can be used to differentiate 11 fish species. These data are a solid basis for the second step to create a Fish Chip for approximately 50 fish species relevant in marine environmental and fisheries research, as well as control of fisheries products.en_US
dc.identifier.doi10.1007/s10126-007-9068-3
dc.identifier.endpage217en_US
dc.identifier.issn1436-2228
dc.identifier.issn1436-2236
dc.identifier.issue2en_US
dc.identifier.pmid18270778en_US
dc.identifier.scopus2-s2.0-40449135996en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage207en_US
dc.identifier.urihttps://doi.org/10.1007/s10126-007-9068-3
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9549
dc.identifier.volume10en_US
dc.identifier.wosWOS:000253755800012en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMarine Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDNA chipsen_US
dc.subjectspecies identificationen_US
dc.subjectcapture oligonucleotideen_US
dc.subjectpiscesen_US
dc.titleDNA microarrays for identifying fishesen_US
dc.typeArticleen_US

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