Decreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses

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dc.authoridIli, Pinar/0000-0002-3107-1798
dc.authoridBODU, Mustafa/0000-0002-8432-7347
dc.authoridSari, Fikret/0000-0002-6141-0690
dc.authoridOzkan, Huseyin/0000-0001-5753-8985
dc.authoridOZTURK, Ali Erdem/0000-0002-3053-4615
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorKeskin, Nazan
dc.contributor.authorIli, Pinar
dc.contributor.authorBodu, Mustafa
dc.contributor.authorAkalin, Pinar Peker
dc.contributor.authorOzturk, Ali Erdem
dc.contributor.authorOzkan, Huseyin
dc.date.accessioned2024-09-18T20:55:38Z
dc.date.available2024-09-18T20:55:38Z
dc.date.issued2020
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThis study aimed to evaluate the comparative effects of taxifolin hydrate and trehalose on the quality of frozen-thawed ram spermatozoa for the first time. Ejaculates collected from six mature rams were pooled, and divided to eight equal aliquots to extend them with different concentrations of glycerol (%5 and %3), taxifolin hydrate (10, 100, and 500 mu M), and trehalose (60 mM) as eight groups (G5T0, G5T10, G5T100, G5T500, G3T0, G3T10, G3T100, and G3T500). After freeze-thawing process of cryopreservation, microscopic and oxidative stress parameters, and gene expression levels were investigated for understanding of possible impacts of taxifolin hydrate and trehalose. The study showed that G3T10 resulted in the highest post-thawed viability and mitochondrial activity. Moreover, all extenders with taxifolin hydrate reduced DNA fragmentation in comparison to G5T0, but DNA damage was prevented at the highest rate in presence of G5T10. The level of LPO significantly decreased in the groups G5T500 and G3T100, and the expression levels of NQO1, GCLC, and GSTP1 genes significantly increased in the groups G5T100, G5T500, G3T10, and G3T100 compared to the group G5T0. Finally, co-supplementation of tris-based extender having 3% glycerol with 60 mM trehalose and 10 mu M taxifolin hydrate in cryopreservation extender may be recommended to improve the quality of post-thawed ram spermatozoa. However, further in vivo and in vitro studies are suggested to evaluate fertility rates of frozen-thawed ram spermatozoa co-supplemented with trehalose and taxifolin hydrate.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [114O642]en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project no: 114O642). Two preliminary drafts of this study were presented as oral presentations at the 16th International Symposium, Prospects for the 3rd Millennium Agriculture in 2017 and at the 4th Symposium on EuroAsian Biodiversity (SEAB-2018) in 2018. The present version of the study was expanded by obtaining new information and data.en_US
dc.identifier.doi10.1016/j.cryobiol.2020.09.001
dc.identifier.endpage29en_US
dc.identifier.issn0011-2240
dc.identifier.issn1090-2392
dc.identifier.pmid32890464en_US
dc.identifier.scopus2-s2.0-85090751385en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage19en_US
dc.identifier.urihttps://doi.org/10.1016/j.cryobiol.2020.09.001
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11972
dc.identifier.volume96en_US
dc.identifier.wosWOS:000579825400003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofCryobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRam semenen_US
dc.subjectCryopreservationen_US
dc.subjectTaxifolin hydrateen_US
dc.subjectTrehaloseen_US
dc.subjectSperm parametersen_US
dc.subjectOxidative stressen_US
dc.subjectAntioxidantsen_US
dc.subjectGene expressionen_US
dc.titleDecreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analysesen_US
dc.typeArticleen_US

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