Detection of phytoplasmas in Rubus spp. by microscopy and molecular techniques in Turkey

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dc.authoridMUSETTI, Rita/0000-0001-8459-0869
dc.authoridERMACORA, Paolo/0000-0003-0757-7956
dc.contributor.authorSertkaya, G
dc.contributor.authorOsler, R
dc.contributor.authorMusetti, R
dc.contributor.authorErmacora, P
dc.contributor.authorMartini, M
dc.date.accessioned2024-09-18T20:15:01Z
dc.date.available2024-09-18T20:15:01Z
dc.date.issued2004
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description10th International Symposium on Small Fruit Virus Diseases -- JUL 21-25, 2003 -- Valencia, SPAINen_US
dc.description.abstractSamples (shoots and leaves) were collected in August 2002 from symptomatic plants in two cultivated blackberry (Rubus spp.) fields and from wild blackberry (Rubus fruticosus L.) plants growing at the border of citrus orchards in Adana, Hatay and Icel provinces in Eastern Mediterranean Region of Turkey. Blackberry plants were observed to show symptoms consisting of numerous short, weak, thin and erect shoots arising from single buds. These shoots produce minute leaves and confer upon the plants a bushy appearance. Cuttings of symptomatic plants were rooted in pots and kept in insect-proof growing room. Small bits of stems taken from symptomatic plants (2 cultivated and 7 wild blackberries) were stained with DAPI and examined with an epifluorescence microscope. Two DAPI-positive samples were also prepared for electron microscopy. Ultra-thin sections of midribs were then examined under a Philips CM10 transmission electron microscope (TEM). After DAPI staining, typical fluorescence was detected in sieve tubes of all symptomatic plants. In ultra-thin sections, phytoplasma bodies of different sizes and shapes occurred in the sieve tubes of midribs. According to symptoms observation of diseased plants, to DAPI staining and TEM analyses, it is suggested that the causal agent of the disease in Rubus spp. is a phytoplasma. The results obtained from PCR/RFLP analyses confirmed the presence of phytoplasmas in Rubus samples. This is the first report of rubus stunt disease in Turkey. Further studies are necessary to investigate distribution and natural transmission of the disease in cultivated Rubus spp.en_US
dc.description.sponsorshipInst Valenciano Invest igac Agrarias,Consell er Agr Pesca Alimentac Generalitat Valenciana,Minist Educ Cienc Direct Gen invest,Univ Politecnica Valencia,Agcy Valenciana Cienc Tecnol,Inst Biolog Mole Celular Plantas,Soc Espanola Ciencias Hort,Soc Espanol Fitopatol,Durviz,Loewe,Biorebaen_US
dc.identifier.doi10.17660/ActaHortic.2004.656.29
dc.identifier.endpage186en_US
dc.identifier.isbn90-6605-099-3
dc.identifier.issn0567-7572
dc.identifier.issn2406-6168
dc.identifier.issue656en_US
dc.identifier.scopus2-s2.0-84856279743en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage181en_US
dc.identifier.urihttps://doi.org/10.17660/ActaHortic.2004.656.29
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9388
dc.identifier.wosWOS:000226753200029en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInt Soc Horticultural Scienceen_US
dc.relation.ispartofProceedings of The Tenth International Symposium on Small Fruit Virus Diseasesen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDAPIen_US
dc.subjectPCR/RFLP analysisen_US
dc.subjectphytoplasmaen_US
dc.subjectRubus spp.en_US
dc.subjectTEMen_US
dc.titleDetection of phytoplasmas in Rubus spp. by microscopy and molecular techniques in Turkeyen_US
dc.typeConference Objecten_US

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