Effects of preservation methods on the ? 13c and ? 15n signatures in muscle tissues of two freshwater fish species

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dc.authorscopusid8696138500
dc.authorscopusid6701444435
dc.authorscopusid14013909100
dc.authorscopusid6505632042
dc.authorscopusid8380888200
dc.authorscopusid57211450814
dc.authorscopusid57211450713
dc.contributor.authorAkin, Şenol
dc.contributor.authorŞahin, Cemalettin
dc.contributor.authorTuran, Davut
dc.contributor.authorGözler, Ahmet Mutlu
dc.contributor.authorVerep, Bülent
dc.contributor.authorBozkurt, Ahmet
dc.contributor.authorÇelik, Kemal
dc.date.accessioned2024-09-19T15:43:49Z
dc.date.available2024-09-19T15:43:49Z
dc.date.issued2011
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractStable isotope is a powerful method for characterizing flows of energy through ecosystems. The power of this method, however, may be affected by preservation methods of the samples. We investigated the effects of four common preservatives (salt, formalin, and ethanol and freezing [control] and preservation duration (six and three months) on ? 15N and ? 13C values of two freshwater fish species, Perca fluviatilis (perch) and Blicca bjoerkna (silver bream). Six-month preservation caused little enrichment in ? 15N of both species compared to three month but had almost the same effects on ? 13C values of both species as in three-month preservation. All methods caused significant shifts (enrichment) in ? 15N of both species, and the effects in general were greater in perch (range: 0.28‰-2.19 ‰) than in bream (range: 0.31‰-1.29‰), which suggested that preservative induced shifts in ? 15N was species-specific. The methods caused little enrichment (ethanol-range: 0.03‰-0.26 ‰ bream and 0.30‰-0.48 ‰ perch and salt: 0.18 ‰ perch three month) and depletion (salt-range: 0.03 ‰0.13‰ bream and 0.13‰ perch six month) in ? 13C. Of the preservatives, however formalin had significant but consistent effects on ? 13C in both species (-1.27‰ and -1.25‰) for the entire preservation duration. Preservation-induced shifts in ? 13C were consistent in direction and magnitude for both species. The results suggested that ethanol and salt could be used without correction factor and formalin with correction factor for preservation of samples solely in ? 13C studies.. For the studies requiring use of carbon and nitrogen together, however, ethanol at least six month in preservation may be suitable for storing samples when considering detection of changes less than 2 ‰ is required in ecological applications. © by PSP.en_US
dc.identifier.endpage2426en_US
dc.identifier.issn1018-4619
dc.identifier.issue9:00 ÖÖen_US
dc.identifier.scopus2-s2.0-80053120191en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage2419en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14577
dc.identifier.volume20en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofFresenius Environmental Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBlicca bjoerknaen_US
dc.subjectEthanolen_US
dc.subjectFormalinen_US
dc.subjectFreshwater fishen_US
dc.subjectPerca fluviatilisen_US
dc.subjectPreservationen_US
dc.subjectSalten_US
dc.subjectStable isotopesen_US
dc.titleEffects of preservation methods on the ? 13c and ? 15n signatures in muscle tissues of two freshwater fish speciesen_US
dc.typeArticleen_US

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