Investigation on the effects of Inula viscosa L. on rainbow trout gonad cells induced by lipopolysaccharide

Basit Öğe Kaydı
dc.contributor.authorKucukgul, Azime
dc.contributor.authorKucukgul, Altug
dc.contributor.authorKorkmaz, Tulay Metin
dc.date.accessioned2024-09-18T20:16:53Z
dc.date.available2024-09-18T20:16:53Z
dc.date.issued2021
dc.departmentHatay Mustafa Kemal Ãœniversitesien_US
dc.description.abstractFish cells have been accepted as an alternative to in vivo assay for inflammatory effects of therapeutic experimental systems. To reveal the anti-inflammatory effects of Inula viscosa (IV) fish cell line RTG-2, derived from the gonadal tissue of rainbow trout, was infected with lipopolysaccharide (LPS). Effective concentrations for different concentrations of LPS (1, 5, 10, 20 mu M) and IN (1, 5, 10, 20 mu g ml(-1)) were determined. While the 20 mu M concentration of LPS, which was effectively selected from preliminary tests, caused 27% cell loss, the effective 1 mu g ml-1 concentration of IN caused 1.1% proliferation in the cells when compared to the control group. All pro-inflammatory parameters investigated in LPS-induced RTG-2 cells showed up-regulation, with the highest increase in TNF-alpha gene expression level (11.3 fold changes). Down-regulation was determined in the IN together with LPS administered group and IL-1 beta had the highest effect with 96%. IL-6 protein level decreased by LPS at a rate of 4% and IN together with LPS increased by 9%. The IN exhibited significant efficacy against inflammation caused by LPS. However, further studies are needed to determine pharmacological activity of I. viscosa in details.en_US
dc.description.sponsorshipUniversity of Munzur, Turkey [YLMUB017-22]en_US
dc.description.sponsorshipThanks are due to the University of Munzur, Turkey for funding this research through the Scientific Research Projects Coordination Unit (Project Number-YLMUB017-22).en_US
dc.identifier.issn2311-729X
dc.identifier.issn2311-3111
dc.identifier.issue1en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9794
dc.identifier.volume9en_US
dc.identifier.wosWOS:000644854500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherBdfishen_US
dc.relation.ispartofJournal of Fisheriesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEndotoxinen_US
dc.subjectInula viscosaen_US
dc.subjectinflammationen_US
dc.subjectrainbow trout gonad cell lineen_US
dc.titleInvestigation on the effects of Inula viscosa L. on rainbow trout gonad cells induced by lipopolysaccharideen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ N/A ]
Ä°sim:
Tam Metin / Full Text
Boyut:
1.18 MB
Biçim:
Adobe Portable Document Format
Ä°ndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu