Effect of Ascorbic Acid and Proline Amino Acid Supplementations on Cryosurvival and Fertility Rates of Cryopreserved Honey Bee (Apis mellifera) Semen

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dc.authoridYALCIN, Oguz Kaan/0000-0002-5508-9861
dc.contributor.authorGuel, Aziz
dc.contributor.authorYildiz, Cengiz
dc.contributor.authorCetin, Nurdan Coskun
dc.contributor.authorYalcin, Oguz Kaan
dc.date.accessioned2024-09-18T19:54:36Z
dc.date.available2024-09-18T19:54:36Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThis study investigated the effect of ascorbic acid (vitamin C) and proline amino acid alone or together on the quality and fertility of frozen/thawed honey bee spermatozoa. The experiments were designed to compare a single ascorbic acid, a single proline amino acid, and different combinations of ascorbic acid with proline amino acid on the cryopreservation of honey bee semen based on sperm motility, viability, intact membrane (hypo-osmotic swelling test), and fertility rates. Eight cryopreserved study groups comprised Control II with no supplement, along with groups with ascorbic acid (2 mg), proline 25 mM, proline 50 mM, proline 100 mM, and combination groups of both ascorbic acid (2 mg) and proline 25 mM, proline 50 mM, and lastly proline 100 mM groups, respectively. Using 50 mM proline in the tested groups had the greatest impact on sperm motility, viability, the percentage of spermatozoa with intact membrane, and fertility. The cryopreservation process caused a gradual decrease in motility, viability, intact membrane (p < 0.05), and fertility rates (p < 0.01) in all the tested research groups as against the fresh semen control group. Successful honey bee sperm cryopreservation and fertility are achievable when using an appropriate sperm freezing protocol and antioxidant. Proline amino acid as an antioxidant in semen extender had a more beneficial influence on sperm quality parameters and fertility. The success of cryopreservation with antioxidants is related to the chosen antioxidant in a dose-dependent manner.en_US
dc.description.sponsorshipCommission of Scientific Researches Projects, the Hatay Mustafa Kemal University, Turkey [18.M.051]en_US
dc.description.sponsorshipThis study was supported by the Commission of Scientific Researches Projects (Project No.: 18.M.051), the Hatay Mustafa Kemal University, Turkey.en_US
dc.identifier.doi10.1089/bio.2021.0077
dc.identifier.endpage556en_US
dc.identifier.issn1947-5535
dc.identifier.issn1947-5543
dc.identifier.issue6en_US
dc.identifier.pmid35020446en_US
dc.identifier.scopus2-s2.0-85144589652en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage551en_US
dc.identifier.urihttps://doi.org/10.1089/bio.2021.0077
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7821
dc.identifier.volume20en_US
dc.identifier.wosWOS:000745301100001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofBiopreservation and Biobankingen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectantioxidanten_US
dc.subjectApis melliferaen_US
dc.subjectcryopreservationen_US
dc.subjectfertilityen_US
dc.subjectsemenen_US
dc.titleEffect of Ascorbic Acid and Proline Amino Acid Supplementations on Cryosurvival and Fertility Rates of Cryopreserved Honey Bee (Apis mellifera) Semenen_US
dc.typeArticleen_US

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