Transformation of recalcitrant melon (Cucumis melo L.) cultivars is facilitated by wounding with carborundum

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dc.authoridGaba, Victor/0000-0001-5405-9946
dc.contributor.authorÇürük, S
dc.contributor.authorÇetiner, S
dc.contributor.authorElman, C
dc.contributor.authorXia, X
dc.contributor.authorWang, Y
dc.contributor.authorYeheskel, A
dc.contributor.authorZilberstein, L
dc.date.accessioned2024-09-18T21:01:56Z
dc.date.available2024-09-18T21:01:56Z
dc.date.issued2005
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractTransformation of the recalcitrant melon (Cucumis melo L.) cultivars Kirkagac 637 and Noi Yarok was accomplished by wounding cotyledon explants by vortexing with carborundum prior to inoculation with Agrobacterium tumefaciens. The addition of silver nitrate to the regeneration-selection medium reduced the transformation efficiency, as the percentage of the explants forming putative transgenic calli and bud-like protuberances was decreased and no transgenic shoots were produced. Chimeric transgenic plants were obtained after the regeneration of putatively transformed callus, bud-like protuberances, buds and shoots on selective medium with kanamycin. The treatments producing the most buds or shoots from explants after 30-40 days of cultivation were the most successful for the production of transgenic plants. Only treatments where explants were vortexed with carborundum produced transgenic melon shoots of either cultivar. Subculture every 18-20 days on fresh regeneration-selection medium containing 50 mg/L kanamycin after either a relatively high (100 mg/L) or low level (50 mg/ L) of kanamycin in the first regeneration-selection medium was necessary for the successful transformation of cultivar Kirkagaq 637. These techniques are now being used in breeding programs for the production of melon lines bearing resistances to zucchini yellow mosaic virus and cucumber mosaic virus, important viruses limiting agricultural production.en_US
dc.identifier.doi10.1002/elsc.200520069
dc.identifier.endpage177en_US
dc.identifier.issn1618-0240
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-20944441666en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage169en_US
dc.identifier.urihttps://doi.org/10.1002/elsc.200520069
dc.identifier.urihttps://hdl.handle.net/20.500.12483/13084
dc.identifier.volume5en_US
dc.identifier.wosWOS:000228786500009en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofEngineering in Life Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAgrobacterium-Mediated Transformationen_US
dc.subjectCoat Protein Geneen_US
dc.subjectHelianthus-Annuus Len_US
dc.subjectShoot Regenerationen_US
dc.subjectMicroprojectile Bombardmenten_US
dc.subjectBeta-Glucuronidaseen_US
dc.subjectTransgenic Plantsen_US
dc.subjectExpressionen_US
dc.subjectTumefaciensen_US
dc.subjectEfficiencyen_US
dc.titleTransformation of recalcitrant melon (Cucumis melo L.) cultivars is facilitated by wounding with carborundumen_US
dc.typeArticleen_US

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