HPV-DNA testing and Ki-67 immunocytochemistry in liquid based cervical cytology in prostitute women

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dc.contributor.authorAtik, Esin
dc.contributor.authorOtlu, Baris
dc.contributor.authorCetin, Meryem
dc.contributor.authorTok, Devrim
dc.contributor.authorDurmaz, Riza
dc.date.accessioned2024-09-18T20:26:50Z
dc.date.available2024-09-18T20:26:50Z
dc.date.issued2006
dc.departmentHatay Mustafa Kemal Ãœniversitesien_US
dc.description.abstractHPV causes several changes in the function of host genes, and these interactions cause deregulation of the cell cycle manifested by abnormal expression of cell cycle associated proteins, such as Ki-67. The detection of Ki-67 can play a role in screening and diagnosis of HPV infection with risk of progression towards dysplasia and carcinoma. To show this relation in prostitute women, cervical cells were collected in the PapSpin Collection Fluid. A starting volume of 1000 mu l for each sample, and a 200 mu l cell suspension were used to prepare each sample for thin layer liquid based cytology and then they were stained by Papanicolaou method. The cytological results were classified according to the Bethesda 2001 system. From the remaining cell suspension of 800 mu l, a 400 mu l sample was used for HPV-DNA detection by PCR, a 50 mu l alliquot was used to make thin layer preparations for immunocytochemistry. Single antigen staining was performed with Ki-67 protein. Cells were considered immunopositive if the nuclei were stained. All cells in one high power field (x400) were counted, and the fraction of immunopositive cells on the slide was calculated. This fraction was expressed as the number of positive cells per 1000 cells to facilitate comparisons of differential cell counts. HPV types 6 and 32 in the study, and HPV types 6 and 51 in the control group were detected. The mean Ki-67 values were 2.7 +/- 1.2 and 3.6 +/- 4.1 in HPV positive and negative cases respectively. There was a positive correlation only with nuclear changes and HPV positivity (x(2) = 28.8, p < 0.001). There was not any significant correlation between HPV or Ki-67 and leukocytosis. An association with HPV and contraception, smoking, and concurrent genital infection was not found. The prevalence of HPV types in different geographical locations and races may indicate different etiologies of cervical cancer. Our results suggest that Ki-67 immunocytochemistry is not useful as a surrogate marker for HPV types of 6, 32 and 51.en_US
dc.identifier.endpage173en_US
dc.identifier.issn1018-5615
dc.identifier.issn1309-5730
dc.identifier.issue3en_US
dc.identifier.startpage167en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10559
dc.identifier.volume22en_US
dc.identifier.wosWOS:000217409000004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherDe Gruyter Open Ltden_US
dc.relation.ispartofTurkish Journal of Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHPVen_US
dc.subjectKi-67en_US
dc.subjectthin layer liquid based cytologyen_US
dc.subjectimmunocytochemistryen_US
dc.titleHPV-DNA testing and Ki-67 immunocytochemistry in liquid based cervical cytology in prostitute womenen_US
dc.typeArticleen_US

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