The Role of Interferences in the Increasing Incidence of Vitamin D Deficiency

Basit Öğe Kaydı
dc.authoridKIRHAN, IDRIS/0000-0001-6606-6078
dc.contributor.authorGonel, Ataman
dc.contributor.authorKirhan, Idris
dc.contributor.authorKoyuncu, Ismail
dc.contributor.authorBayraktar, Nihayet
dc.contributor.authorKaradag, Mujgan Ercan
dc.contributor.authorKaradag, Mehmet
dc.date.accessioned2024-09-18T19:47:57Z
dc.date.available2024-09-18T19:47:57Z
dc.date.issued2020
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractObjective: Lipemia is one of the causes of interference in immunoassay and LC-MS/MS methods. Increased prevalence of vitamin D deficiency in the US, where obesity is gradually increasing, raises the suspicion that high levels of fat diet and blood lipid levels interfere with vitamin D measurement results. The focus of this study was to investigate the effect of blood lipid profiles on vitamin D results and prevent the matrix effect. Material and Methods: In this study, 25OH vitamin D3 (25OHD3) levels of 100 samples consecutively accepted to biochemistry laboratory regardless of age and sex were measured by the LC-MS/MS method, and each sample was restudied after 1/10 dilution. After dilution restudy, two groups were obtained-group 1 (results deviating below 20%) and group 2 (results deviating above 20%)-and the difference between the groups was investigated. There were 79 patients in group 1 and 21 patients in group 2. In our study, lipid profiles (triglyceride, total cholesterol, HDL, LDL) from the same samples of consecutive vitamin D patients were studied. Results: It was observed that the triglyceride, total cholesterol HDL, LDL, and 25OHD3 measurements of group 1 and group 2 were similar (p > 0.05). While the mean vitamin D value in the second group was 9.94 +/- 7.85, the mean vitamin D value after dilution was measured as 39.23 +/- 18.13 and was statistically significant. 25OHD3 concentrations of 21 patients out of 100 were found to be falsely low. Measurements were repeated to confirm the results. Conclusion: The matrix effect caused by exogenous and endogenous interferences in the blood could be a hidden factor increasing the prevalence of vitamin D deficiency by causing falsely low 25OHD3 values. Suspicious results should be remeasured by a dilution study.en_US
dc.identifier.doi10.2174/1871530320666200604160208
dc.identifier.endpage1308en_US
dc.identifier.issn1871-5303
dc.identifier.issn2212-3873
dc.identifier.issue8en_US
dc.identifier.pmid32496995en_US
dc.identifier.scopus2-s2.0-85090625130en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1303en_US
dc.identifier.urihttps://doi.org/10.2174/1871530320666200604160208
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7247
dc.identifier.volume20en_US
dc.identifier.wosWOS:000581548400015en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBentham Science Publ Ltden_US
dc.relation.ispartofEndocrine Metabolic & Immune Disorders-Drug Targetsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject25OHD3en_US
dc.subjectmismeasurementen_US
dc.subjectLC-MS/MSen_US
dc.subjectmatrix effecten_US
dc.subjectdilutionen_US
dc.subjectfalse resulten_US
dc.titleThe Role of Interferences in the Increasing Incidence of Vitamin D Deficiencyen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu