Pathomorphological and immunohıstochemical evaluation of unilateral progressive Mooren's ulcer in a cat

Basit Öğe Kaydı
dc.authorscopusid30867562400
dc.authorscopusid57111967800
dc.authorscopusid54968640300
dc.authorscopusid56404386200
dc.authorscopusid6602539692
dc.contributor.authorAlcigir, Mehmet Eray
dc.contributor.authorKutlu, Tuncer
dc.contributor.authorErgin, Irem
dc.contributor.authorKarabulut, Sefika
dc.contributor.authorAlcigir, Gunay
dc.date.accessioned2024-09-19T15:45:26Z
dc.date.available2024-09-19T15:45:26Z
dc.date.issued2018
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground: Mooren's ulcer is a chronic and painful ulceration of the cornea. It begins progressively in the periphery and spread centrally in cornea. In human, it is seen uniaterally in most of cases. Mooren ulcer has not been reported in any kind of animals up to now. Although its aetiology is not completely enlighted, it has been suspected of the inflammatory reaction against injuries-microbiological and immun mediated effects. Immun response in presence of accumulation of immune complexes into the limbal vessels.As a result of the deficit in the regulatory mechanism because the number of suppressor cells control over B and T lymphocytes, These situations can result in a progressive tendancy to inflammation because the production of autoantibodies and/or lymphokine from cytotoxic T-lymhocytes creates an immune-mediated vasculitis. Numerous immigrant inflammatory cells and proteins are evaded from vessels. After triggering inflammatory cells and releasing of meditors, corneal vascularization, scar tissue and re-epithelization develop. This regenerative-reperative process plays an important role during post-inflammatory process. Case: In this case, it was aimed to detect pathomorphological structure and immunologic relations in progressive Mooren's ulcer (MU). A 1 year-old mix breed cat was submitted to clinic with complaints of progressive painful and eyesight loss in left eye. There were 1 cm-ulceration, opacification and old haemorrhagic areas at peripheral cornea. Histopathologically, there was wide ulceration including all layers of corneal epithelium and particularly vacuolar degeneration at suprabasal cells. In corneal stroma, numerous neutrophiles and mononuclear cells were infiltrated. Neovascularisation and fibrosis beginning from limbus were also present. This fibrotic progress was confirmed by Masson's trichrome staining method. Immunohistochemically, Cytokeratin 3 (CK3) and cytokeratin 12 (CK12) positivities showing regenerative activity of suprabasal and basal cells were not widespread. Epidermal Growth Factor (EGF) positivities were generally weak in epithelial cells. In stroma, moderate vimentin positivities were detected proliferated in fibrocytes originating from limbus. ?1-antichymotrypsin (A1AC) was mildly reacted in neutrophiles. CD3 and CD4 confirmed the presence of regulatory and helper T lymphocytes. CD3 and CD8 marked cytotoxic T lymphocytes and CD20 marked B lymphocytes in inflammatory areas. CD34 were also positive in peripheral corneal stem cells derived from limbal basal epitheliums in partly regenerated area. CD57 positivity in T lymphocytes and NK cells and CD68 positivity in macrophages were attended to the area. Discussion: CD1a positivity in T lymphocytes proved mediating lipid and microbial origin glycolipid antigens. TUNEL reactions showing DNA in situ fragmentation were present in the destructive and aging epithelial cells at periphery. In conclusion, the case has been found as unique in terms of its immunohistochemical characterization. The markers show that CD1a and CD68 expressions follow different progress in animals unlike in humanbeings even though the ulcer of pathogenetic mechanism is found identical to humanbeings.. The roles of CD20 and CD57 markers have potential roles in this ulcer. It is also concluded that insufficient epithelial regeneration, fibrosis, inflammation and apoptosis showed progressive Mooren's ulcers having possibly microbial origin. © 2018 Universidade Federal do Rio Grande do Sul. All rights reserved.en_US
dc.identifier.doi10.22456/1679-9216.86588
dc.identifier.issn1678-0345
dc.identifier.scopus2-s2.0-85077641317en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://doi.org/10.22456/1679-9216.86588
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14689
dc.identifier.volume46en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUniversidade Federal do Rio Grande do Sulen_US
dc.relation.ispartofActa Scientiae Veterinariaeen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCaten_US
dc.subjectImmunohistochemistryen_US
dc.subjectMooren's ulceren_US
dc.subjectPathomorphologyen_US
dc.titlePathomorphological and immunohıstochemical evaluation of unilateral progressive Mooren's ulcer in a caten_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu