Immunohistochemical Analysis of MCC, TARC and CD 104 Antigens in Human Lung Tissue

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dc.contributor.authorKorkmaz, Metin
dc.contributor.authorAkkus, Murat
dc.contributor.authorYildirim, Ayse
dc.contributor.authorBaran, Ozlem Pamukcu
dc.contributor.authorAktas, Ayfer
dc.date.accessioned2024-09-18T20:11:31Z
dc.date.available2024-09-18T20:11:31Z
dc.date.issued2010
dc.departmentHatay Mustafa Kemal Ãœniversitesien_US
dc.description.abstractObjective: The aim of this study was to investigate the the distribution of CMA 1 protein (MCC), CCL17 protein (TARC) and Integrin beta 4 (CD 104) monoclonal antibodies in normal adult human lung tissue. Material and Methods: In this study we examined the lung biopsy specimens obtained from the adult patients who underwent operations for different reasons in the Department of Lung Surgery at Dicle University Hospital, Diyarbakir, Turkey. The tissue samples were immediately frozen in liquid nitrogen at -196 degrees C. The samples were immunostained by indirect immune peroxidase technique. As primary antibodies, MCC, TARC and CD104 were used. As secondary antibodies, 1:200 rabbit anti - mouse IgG peroxidase diluted in PBS/BSA and 1:100 normal human serum solution were used. Negative control staining was performed using irrelevant mouse monoclonal antibodies omitting the primary antibody step. Sections were examined and photographed by Olympus BH2 light microscope. Results: We observed moderate reaction with vascular endothelial cells and with vascular smooth muscle cells with MCC which was aimed to show overall the presence of human lung mast cells. TARC, which was aimed to express the follicular dentritic cells, moderately reacted with follicular dentritic cells, bronchial smooth muscle cells and vascular endothelial cells. CD104, which is the integrin beta 4 subunit that plays an important role in the adhesion of epithelium to basement membranes, moderately reacted with vascular endothelial cells and mainly reacted with the follicular dentritic cells of bronchi. Conclusion: In our study, it was defined that the expressions of these molecules have important role in understanding the function of human lung tissue. We reached the conclusion that our study, by presenting the difference between the normal lung tissue and pathologic lung tissue with expression the related proteins immunohistochemically may constitute preliminary findings for excluding the diseases like interstisial lung disease or chronic obstructive lung disease for the aim of. Our preliminary findings may shed light on future studies.en_US
dc.identifier.doi10.5336/medsci.2009-13920
dc.identifier.endpage1978en_US
dc.identifier.issn1300-0292
dc.identifier.issn2146-9040
dc.identifier.issue6en_US
dc.identifier.startpage1971en_US
dc.identifier.urihttps://doi.org/10.5336/medsci.2009-13920
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8917
dc.identifier.volume30en_US
dc.identifier.wosWOS:000287053500026en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherOrtadogu Ad Pres & Publ Coen_US
dc.relation.ispartofTurkiye Klinikleri Tip Bilimleri Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCMA1 protein, humanen_US
dc.subjectCCL17 protein, humanen_US
dc.subjectIntegrin beta4en_US
dc.titleImmunohistochemical Analysis of MCC, TARC and CD 104 Antigens in Human Lung Tissueen_US
dc.typeArticleen_US

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