Thermolongibacillus altinsuensis gen. nov., sp nov and Thermolongibacillus kozakliensis sp nov., aerobic, thermophilic, long bacilli isolated from hot springs

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dc.authoridColeri Cihan, Arzu/0000-0002-7289-6251
dc.contributor.authorCihan, Arzu Coleri
dc.contributor.authorKoc, Melih
dc.contributor.authorOzcan, Birgul
dc.contributor.authorTekin, Nilgun
dc.contributor.authorCokmus, Cumhur
dc.date.accessioned2024-09-18T21:05:12Z
dc.date.available2024-09-18T21:05:12Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractTwo novel endospore-forming, aerobic bacilli, strains E173a(T) and E265(T), were isolated from soil and sediment samples from Kozakli and Altinsu hot springs, Nevsehir (Turkey). Their young cells in the exponential phase of growth were motile, Gram-stain-positive, straight rods, 0.6-1.1x3.0-8.0 mu m in size, but they became strikingly long, approximately 0.6-1.2 by 9.0-35.0 mu m, after the stationary phase of growth. Cells varied in tests for oxidase, and had a weakly positive reaction for catalase. Both strains could grow between 40 and 70 degrees C, with optimal growth at 60 degrees C (E173a(T)) and 55 degrees C (E265(T)). Growth occurred within the range pH 5.0-11.0 with optimal growth at pH 9.0 (E173a(T)) and pH 8.5 (E265(T)). Strain E173a(T) grew within a salinity range from 0 to 1.5 % (w/v) NaCl with optimal growth at 0.5%, while strain E265(T) grew within the range 0-5.0% (w/v), with an optimum at 3.0%. The new isolates differed from each other in some phenotypic and chemotaxonomic characters as well as repetitive extragenic palindromic element PCR (rep-PCR) fingerprints. 16S rRNA gene sequence similarities suggested distant relationships with other members of the family Bacillaceae (<95.8%), although the two strains showed 97.5% sequence similarity between them, and had 55% relatedness by DNA-DNA hybridization. The DNA G+C contents were 44.8 (E173a(T)) and 43.5 mol% (E265(T)). Moreover, the chemotaxonomic data of E173a(T) and E265(T) [presence of low amounts of meso-diaminopimelic acid, A1 gamma to A1 gamma' cross-linkage types in peptidoglycan, fatty acids including iso-C-15:0 (>60%), iso-C-17:0 and C-16:0] supported the consideration of these isolates as members of a novel genus. Based upon phenotypic, phylogenetic and chemotaxonomic characteristics, it is proposed that new isolates represent a novel genus, Thermolongibacillus gen. nov., with two novel species: Thermolongibacillus altinsuensis sp. nov. (type strain E265(T)=DSM 24979(T)=NCIMB 14850(T)) and Thermolongibacillus kozakliensis sp. nov. (type strain E173a(T)=DSM 24978(T)=NCIMB 14849(T)).en_US
dc.description.sponsorshipScientific Research Project Office of Ankara University [11B4240003]en_US
dc.description.sponsorshipThis research was supported by the Scientific Research Project Office of Ankara University with the project number 11B4240003.en_US
dc.identifier.doi10.1099/ijs.0.053280-0
dc.identifier.endpage197en_US
dc.identifier.issn1466-5026
dc.identifier.issn1466-5034
dc.identifier.pmid24048871en_US
dc.identifier.scopus2-s2.0-84891892372en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage187en_US
dc.identifier.urihttps://doi.org/10.1099/ijs.0.053280-0
dc.identifier.urihttps://hdl.handle.net/20.500.12483/13442
dc.identifier.volume64en_US
dc.identifier.wosWOS:000337930400031en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMicrobiology Socen_US
dc.relation.ispartofInternational Journal of Systematic and Evolutionary Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRapid Methoden_US
dc.subjectPhylogenetic Diversityen_US
dc.subjectDeoxyribonucleic-Aciden_US
dc.subjectGeothermal Regionsen_US
dc.subjectDna Hybridizationen_US
dc.subjectAlpha-Glucosidaseen_US
dc.subjectLipid-Compositionen_US
dc.subjectBacteriumen_US
dc.subjectProposalen_US
dc.subjectGeneraen_US
dc.titleThermolongibacillus altinsuensis gen. nov., sp nov and Thermolongibacillus kozakliensis sp nov., aerobic, thermophilic, long bacilli isolated from hot springsen_US
dc.typeArticleen_US

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