Characterization of extended spectrum ?-lactamase ( ESBL)-producing Escherichia coli in Asi (Orontes) River in Turkey
Yükleniyor...
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Iwa Publishing
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, the presence of extended spectrum beta-lactamase (ESBL)-producing Escherichia coli in aquatic environments (the Orontes River and an urban wastewater) was investigated. Fifty-four E. coli strains resistant to cefotaxime were isolated from the river waters and nearby waste water treatment plant and screened for ESBL gene variants, different classes of integrons and sulfonamide resistance genes. The ESBL-producing E. coli strains were further characterized by PhP-typing system, phylogenetic grouping and antimicrobial susceptibility testing. Of the 54 ESBL-producing strains, 14 (25.9%) belonged to four common PhP types and the remaining were of single types. CTX-M type ESBL genes were identified in 68% of the isolates. The most predominant specific CTX-M subtype identified was bla(CTX-M-15) (n=36), followed by bla(CTX-M-1) (n = 1). None of the isolates were SHV and OXA positive. Most of the ESBL positive isolates (n = 37; 68.5%) were harboring sul gene. This study indicates a widespread distribution of CTX-M-15 producing E. coli strains in the surface waters in part of Turkey, suggesting an aquatic reservoir for ESBL genes.
Açıklama
Anahtar Kelimeler
Escherichia coli, extended spectrum beta-lactamases, Orontes River, PhP-typing
Kaynak
Journal of Water and Health
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
15
Sayı
5
