Copper Removal by SCMT II Transgenic and Wild Type Tobacco in Hydroponic System - a Comparative Study

Basit Öğe Kaydı
dc.authoridUYGUR, VELI/0000-0003-3971-7714
dc.authoridArslan, Mehmet/0000-0002-0530-157X
dc.authoridDAGHAN, HATICE/0000-0002-0150-5882
dc.contributor.authorDaghan, Hatice
dc.contributor.authorUygur, Veli
dc.contributor.authorArslan, Mehmet
dc.contributor.authorKoleli, Nurcan
dc.date.accessioned2024-09-18T20:13:20Z
dc.date.available2024-09-18T20:13:20Z
dc.date.issued2012
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractSaccharomyces cerevisiae metallothionein-II (ScMT-II) genes expressed in Nicotiana tabacum and wild tobacco were grown for 14 days in growth chamber as hydroponics containing 0, 5, and 10 Cu mg L-1 to determine their Cu accumulation ability and to study Cu compartmentation in shoots and roots. Wild type (Nicotiana tabacum L., cv. Petite Havana SRI) and transgenic tobacco (p-S-ScMTII) plants wilted severely, showed stunted root growth with structural deformation. Shoot dry weight, Cu, and free sulphydryl (-SH) groups showed significant differences between transgenic and wild type tobacco plants. Copper accumulation in roots was 7.78 and 10.8 g kg(-1) for transgenic and wild type tobacco, respectively. The study revealed that despite high Cu accumulation in roots, its translocation from roots to shoots was inhibited probably due to blockage and dieback in the root collar. It can be concluded that the main cause of failure in MTII gene induced Cu accumulation in shoots was collapse of root collars due to Cu toxicity. Thus it was not possible to judge the real effect of the MT gene.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [108-O-161]en_US
dc.description.sponsorshipWe would like to thank Prof. Dr. Rainer Fisher (RWTH Aachen, Biology VII Department) for supplying tobacco plants material. This work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) as a research project (Project No: 108-O-161).en_US
dc.identifier.endpage1197en_US
dc.identifier.issn0034-7752
dc.identifier.issue12en_US
dc.identifier.scopus2-s2.0-84873383959en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1193en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9106
dc.identifier.volume63en_US
dc.identifier.wosWOS:000313229100001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherChiminform Data S Aen_US
dc.relation.ispartofRevista De Chimieen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCopperen_US
dc.subjectrooten_US
dc.subjectshooten_US
dc.subjectcompartmentationen_US
dc.subjecttransgenicsen_US
dc.subjectphytoremediationen_US
dc.titleCopper Removal by SCMT II Transgenic and Wild Type Tobacco in Hydroponic System - a Comparative Studyen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu