Cryopreservation of scaly carp (Cyprinus carpio) sperm: effect of different cryoprotectant concentrations on post-thaw motility, fertilization and hatching success of embryos

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dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.contributor.authorYavas, Ilker
dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYildiz, Cengiz
dc.date.accessioned2024-09-18T20:56:51Z
dc.date.available2024-09-18T20:56:51Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe aim of the present study was to determine the effect of various cryoprotectants on post-thaw sperm quality and fertilizing capacity of cryopreserved scaly carp (Cyprinus carpio) semen. The present study focused on freezing of scaly carp sperm utilizing a practical and inexpensive protocol for aquaculture. Semen was diluted with Kurokura's extender composing 3.6 g/l NaCl, 10 g/l KCl, 0.22 g/l CaCl2, 0.08 g/l MgCl2 and 0.2 g/l NaHCO3. The extender contained three different cryoprotectants (DMSO, DMA and egg yolk) at ratios of 5, 10 and 15 %. Semen was placed into 0.25-ml straws and exposed to liquid nitrogen vapor (-120 A degrees C) using an insulated box with an adjustable tray for 10 min and then plunged into liquid nitrogen (-196 A degrees C) tank. The thawing process was performed in a water bath at 40 A degrees C for 10 s. The results indicated that type of cryoprotectants and their concentrations are rather effective in scaly carp sperm cryopreservation on post-thaw sperm quality, while they are very important in order to obtain high fertilization rates. The highest fertilization rate was determined as 96.4 +/- A 0.15 % with 15 % egg yolk, while the highest hatching rate was determined as 99.3 +/- A 0.80 with 15 % DMA. In conclusion, the applied cryopreservation method for scaly carp sperm is suitable to fertilize high amounts of eggs.en_US
dc.description.sponsorshipScientific Research Institute of Mustafa Kemal University [MKU-08-E-0206]en_US
dc.description.sponsorshipThis research was funded by a grant from Scientific Research Institute of Mustafa Kemal University (MKU-08-E-0206). The authors would like to thank the staff of the State Hydraulic Works (SHW) Fish Production Station in Adana (Turkey) for their technical assistance. Also, the valuable comments and suggestions from anonymous reviewers are deeply thanked.en_US
dc.identifier.doi10.1007/s10499-013-9698-6
dc.identifier.endpage148en_US
dc.identifier.issn0967-6120
dc.identifier.issn1573-143X
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84892433105en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage141en_US
dc.identifier.urihttps://doi.org/10.1007/s10499-013-9698-6
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12127
dc.identifier.volume22en_US
dc.identifier.wosWOS:000329939600014en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofAquaculture Internationalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectScaly carpen_US
dc.subjectCyprinus carpioen_US
dc.subjectCryoprotectanten_US
dc.subjectCryopreservationen_US
dc.subjectMotilityen_US
dc.subjectFertilizationen_US
dc.subjectHatchingen_US
dc.titleCryopreservation of scaly carp (Cyprinus carpio) sperm: effect of different cryoprotectant concentrations on post-thaw motility, fertilization and hatching success of embryosen_US
dc.typeArticleen_US

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