Molecular detection of ‘Candidatus Phytoplasma mali’ associated with virescence in Narcissus tazetta in Turkey

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dc.authorscopusid56399352200
dc.authorscopusid57194397684
dc.authorscopusid58408864100
dc.authorscopusid14831197300
dc.authorscopusid13611411000
dc.authorscopusid55945299100
dc.authorscopusid55608982400
dc.contributor.authorBoztas, Kadir
dc.contributor.authorKocabag, Hamide Deniz
dc.contributor.authorDerecik, Kayhan
dc.contributor.authorGazel, Mona
dc.contributor.authorSipahioglu, Hikmet Murat
dc.contributor.authorCaglayan, Kadriye
dc.contributor.authorTulum, Isil
dc.date.accessioned2024-09-19T15:41:19Z
dc.date.available2024-09-19T15:41:19Z
dc.date.issued2023
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn the winter 2019 virescence, flower sterility, and stunting symptoms were observed in daffodil plants in Mersin province of Turkey. To detect the presence of phytoplasmas ten plants showing symptoms and ten asymptomatic plants were assayed by PCR analysis. The nested-PCR analysis of extracted DNA with universal primer pairs amplifying 16S phytoplasma ribosomal gene were positive for 9 out of the 10 symptomatic daffodil samples. BLAST analysis of the 16S rDNA sequences, virtual restriction fragment length polymorphism (RFLP) and RFLP analyses confirmed the presence of ‘Candidatus Phytoplasma mali’ in Narcissus tazetta plants. The detected strains of ‘Ca. P. mali’ were sequenced and compared in multiple alignments using sequences in NCBI GenBank. The nucleotide identity among the 16S rDNA gene fragments ranged between 99.26 and 99.91% to ‘Ca. P. mali’, ribosomal subgroup 16SrX-A. Although phytoplasmas in this subgroup have been reported frequently from woody plants, this is the first report of a ‘Ca. P. mali’ strain infecting daffodil plants. © 2023, Technology Society of Basic and Applied Sciences. All rights reserved.en_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (:118C290)en_US
dc.identifier.doi10.5958/2249-4677.2023.00057.9
dc.identifier.endpage114en_US
dc.identifier.issn2249-4669
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85163590442en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage113en_US
dc.identifier.urihttps://doi.org/10.5958/2249-4677.2023.00057.9
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14188
dc.identifier.volume13en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTechnology Society of Basic and Applied Sciencesen_US
dc.relation.ispartofPhytopathogenic Mollicutesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject16SrX-A subgroupen_US
dc.subjectdaffodilen_US
dc.subjectPCR/RFLPen_US
dc.subjectphyllodyen_US
dc.titleMolecular detection of ‘Candidatus Phytoplasma mali’ associated with virescence in Narcissus tazetta in Turkeyen_US
dc.typeArticleen_US

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