Do Fasudil and Y-27632 affect the level of transient receptor potential (TRP) gene expressions in breast cancer cell lines?

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dc.authoridBayraktar, Recep/0000-0002-8227-2055
dc.authoridIzmirli, Muzeyyen/0000-0002-8545-863X
dc.contributor.authorGogebakan, Bulent
dc.contributor.authorBayraktar, Recep
dc.contributor.authorSuner, Ali
dc.contributor.authorBalakan, Ozan
dc.contributor.authorUlasli, Mustafa
dc.contributor.authorIzmirli, Muzeyyen
dc.contributor.authorOztuzcu, Serdar
dc.date.accessioned2024-09-18T20:06:12Z
dc.date.available2024-09-18T20:06:12Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBreast cancer (BC) is the most frequent cancer type in women, and the mortality rate is high especially in metastatic disease. Ion channels such as the transient receptor potential (TRP) channels correlate with malignant growth and cancer progression. Hence, some authors have suggested that the expression levels of TRP channels may be used as a marker in the diagnosis and predicting the prognosis of BC. Also, in some recent studies, targeting TRP channels are suggested as a novel treatment strategy in BC. The aim of this study was to investigate the effect of two Rho-kinase (ROCK) inhibitors, fasudil and Y-27632, on the expression levels of TRP channel genes in breast cancer cell lines (ZR-75-1, MCF7, and MDA-MB-231) and breast epithelial cell line (hTERT-HME1). The expression levels of TRP genes were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). We found that fasudil had reduced the TRPC1, TRPV2 expression levels in the ZR-75-1, MCF7, and MDA-MB-231 cell lines. On the other hand, fasudil and Y-27632 had reduced TRPM6 expression levels in all cell lines. Y-27632 increased the expression levels of TRPC7 in all cell lines. In conclusion, this is the first study demonstrating that the inhibition of ROCK pathway changes the expression levels of some TRP genes. Also, our study has firstly shown that the expression levels of the TRP genes which are suggested as a diagnostic and prognostic biomarker in BC, were changed with the treatment of fasudil and Y-27632.en_US
dc.identifier.doi10.1007/s13277-014-1752-0
dc.identifier.endpage8041en_US
dc.identifier.issn1010-4283
dc.identifier.issn1423-0380
dc.identifier.issue8en_US
dc.identifier.pmid24839003en_US
dc.identifier.scopus2-s2.0-84922394849en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage8033en_US
dc.identifier.urihttps://doi.org/10.1007/s13277-014-1752-0
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8367
dc.identifier.volume35en_US
dc.identifier.wosWOS:000341883500096en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofTumor Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBreast canceren_US
dc.subjectFasudilen_US
dc.subjectTRP genesen_US
dc.subjectY-27632en_US
dc.titleDo Fasudil and Y-27632 affect the level of transient receptor potential (TRP) gene expressions in breast cancer cell lines?en_US
dc.typeArticleen_US

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