Development, Quality, and Production Parameters of In Vitro Embryo in Anatolian Water Buffaloes

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dc.contributor.authorYilmaz, M. A.
dc.contributor.authorKaymaz, M.
dc.contributor.authorErgun, Y.
dc.date.accessioned2024-09-18T19:54:18Z
dc.date.available2024-09-18T19:54:18Z
dc.date.issued2024
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe aim of the study was to determine in vitro embryo development, embryo quality, and in vitro embryo production (IVEP) parameters by using Anatolian water buffaloes (AWB) oocytes. In this study, 184 ovaries of 92 AWB obtained from slaughterhouses were used. The tissue culture medium (TCM-199) was used for in vitro maturation (IVM), Brackett & Oliphant (BO) medium for in vitro fertilization (IVF), and Charles Rosencrans 1 amino acid (CR1aa) medium for in vitro culture (IVC). A total of 395 oocytes (2.15 per ovary) were obtained from the ovaries. Frozen AWB sperm was used for fertilization. The number of cleavages at the 24th hour was 93 out of 302 (30.79%), the number of morulae and compact morula at the 96th hour was 53 out of 302 (17.55%), the number of blastocysts at 7th day was 29 out of 302 (9.60%), and the number of hatched blastocysts were 12 out of 302 (3.97%) on the 8th and 9th days. In the quality assessment of 29 blastocysts recovered on day 7 in IVC, 7 of them (24.13%) were of the code 1 quality, 9 of them (31.03%) were of the code 2 quality, 8 of them (27.50%) were of the code 3 quality, and 5 of them (17.24%) were classified as the code 4 quality. This study provides the first data on in vitro embryo development, embryo quality classification, and embryo production in AWB. As a result, the potential of oocytes AWB for IVEP has been revealed, and a scientific background has been provided for future studies.en_US
dc.description.sponsorshipRepublic of Turkey, Ministry of Agriculture and Forestry, General Directorate of Agricultural Research and Policies [TAGEM/HAYSUD/B/18/A4/P1/260]en_US
dc.description.sponsorshipThis article is summarized from a part of Mehmet Ali Yilmaz's Ph.D. thesis. The authors declared that there was no conflict of interest in the publication of this paper. This study was supported by the Republic of Turkey, Ministry of Agriculture and Forestry, General Directorate of Agricultural Research and Policies project numbered TAGEM/HAYSUD/B/18/A4/P1/260. The authors would like to thank all individuals and organizations who devotedly worked on this difficult project and helped pass this data on to future generations.en_US
dc.identifier.doi10.5398/tasj.2024.47.1.1
dc.identifier.endpage8en_US
dc.identifier.issn2615-787X
dc.identifier.issn2615-790X
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85188989735en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.5398/tasj.2024.47.1.1
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7650
dc.identifier.volume47en_US
dc.identifier.wosWOS:001184390300001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBogor Agricultural Univ, Fac Animal Scienceen_US
dc.relation.ispartofTropical Animal Science Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAnatolian Water buffaloesen_US
dc.subjectdevelopmenten_US
dc.subjectembryoen_US
dc.subjectfertilizationen_US
dc.subjectoocyteen_US
dc.titleDevelopment, Quality, and Production Parameters of In Vitro Embryo in Anatolian Water Buffaloesen_US
dc.typeArticleen_US

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