MOLECULAR EPIDEMIOLOGY AND RISK FACTORS IN EXTENDED-SPECTRUM BETA LACTAMASE POSITIVE AND QUINOLONE-RESISTANT ESCHERICHIA COLI STRAINS ISOLATED FROM URINARY TRACT INFECTIONS
Yükleniyor...
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Carbone Editore
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Introduction: The study was designed to investigate the epidemiology, risk factors, and clonal relationships in extended-spectrum beta lactamase (ESBL)-positive and quinolone resistant Escherichia coli strains isolated from urinary tract infections. Materials and methods: The study included 96 E. coli strains isolated from urine samples and identified as the infection agent. All the strains were ESBL positive and quinolone resistant. ESBL production was evaluated by the double disc synergy test. The clonal proximity of the isolates was determined with Rep-PCR method. Results: Fifty-two (54.2%) of the 96 isolates were isolated from nosocomial infections, and 44 (45.8%) were isolated from community-acquired infections. Three common risk factors for ESBL-positive E.coli infections were identified. The most common risk factor was hospitalization in the last 6 months. The Rep-PCR technique detected 29 clones, of which nine were major clones and 20 were sporadic. Conclusion: The identification of clonal relationships using Rep-PCR can reveal the source of infections and means of dissemination. Community-acquired isolates that cause infections may be associated with hospitalization. Strains colonized in hospital for along time can increase infections in various departments. An increase in the resistance to any drug in any unit of a hospital may be the harbinger of a pandemic. The source of infection and ways of spread can be identified most accurately by the demonstration of clonal relationships.
Açıklama
Anahtar Kelimeler
Escherichia coli, Extended-spectrum beta lactamase, Quinolone resistance, Clonal proximity, Repetitive-sequence-based polymerase chain reaction
Kaynak
Acta Medica Mediterranea
WoS Q DeÄŸeri
Q4
Scopus Q DeÄŸeri
Cilt
31
Sayı
5
