THE EFFECT OF DIFFERENT PRESERVATION MEDIA AND TEMPERATURES ON SPERM QUALITY AND DNA INTEGRITY IN MOUSE CAUDA SPERMATOZOA

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dc.contributor.authorYildiz, Cengiz
dc.contributor.authorCoskun Cetin, Nurdan
dc.contributor.authorYavas, Ilker
dc.contributor.authorYalcin, Oguz Kaan
dc.contributor.authorYilmaz, Firdevs
dc.contributor.authorKaraca, Fikret
dc.date.accessioned2024-09-18T20:13:20Z
dc.date.available2024-09-18T20:13:20Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBACKGROUND: Mouse sperm can be stored for long or short-time periods. Nevertheless long-term storage leds to significantly reduced sperm quality and fertility because of cryodamage. Thus, in the storage of semen in mice, it is necessary to focus on media and temperatures that gives good results in short-term storage. OBJECTIVE: To determine favorable media for short-term storage of mice spermatozoa by evaluating progressive motility, viability, membrane function integrity, acrosome integrity and fragmented DNA rates at various storage temperatures. MATERIALS AND METHODS: Mouse spermatozoa were collected from epididymides of mature CD1 males and samples were stored at 24 degrees C and 4 degrees C for 60 h. RESULTS: Motility, viability and membrane function of mice spermatozoa were greatest when stored in KSOM media. Motility and viability were not different when stored at refrigerator or room temperature in KSOM compared to HTF or PBS mediums for 48 h, but were after 60 h. There wasn't any significant variation in terms of acrosome integrity in different preservation conditions. Fragmented DNA rates were similar in fresh sperm with KSOM and HTF media, while there was higher damage in PBS medium at 60 h. Overall, sperm parameters were affected significantly by the time of storage and type of preservation medium, and PBS extender was not suitable for mice spermatozoa at room and refrigerated temperatures as it caused the lowest progressive motility, viability, membrane function integrity and the highest DNA damage. CONCLUSION: Mice spermatozoa stored in KSOM retained the best sperm quality parameters both 24 degrees C and 4 degrees C for the first 48 h.en_US
dc.description.sponsorshipHMKU BAP [16566]en_US
dc.description.sponsorshipThis research was funded by HMKU BAP (Scientific Research Project: 16566).en_US
dc.identifier.doi10.54680/fr22610110612
dc.identifier.endpage356en_US
dc.identifier.issn0143-2044
dc.identifier.issn1742-0644
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85146136766en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage349en_US
dc.identifier.urihttps://doi.org/10.54680/fr22610110612
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9096
dc.identifier.volume43en_US
dc.identifier.wosWOS:000923631400007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherCryo Lettersen_US
dc.relation.ispartofCryolettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcoolingen_US
dc.subjectDNA integrityen_US
dc.subjectKSOMen_US
dc.subjectmouseen_US
dc.subjectroom temperatureen_US
dc.subjectsperm qualityen_US
dc.titleTHE EFFECT OF DIFFERENT PRESERVATION MEDIA AND TEMPERATURES ON SPERM QUALITY AND DNA INTEGRITY IN MOUSE CAUDA SPERMATOZOAen_US
dc.typeArticleen_US

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