The effect of raffinose and methionine on frozen/thawed Angora buck (Capra hircus ancryrensis) semen quality, lipid peroxidation and antioxidant enzyme activities

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dc.authoridATESSAHIN, Ahmet/0000-0002-1004-2146
dc.authoridYeni, Deniz/0000-0002-9105-5677
dc.authoridTUNCER, Purhan Barbaros/0000-0002-9257-9544
dc.contributor.authorTuncer, Puerhan Barbaros
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorSariozkan, Serpil
dc.contributor.authorSakin, Fatih
dc.contributor.authorYeni, Deniz
dc.contributor.authorCigerci, Ibrahim Hakki
dc.contributor.authorAtessahin, Ahmet
dc.date.accessioned2024-09-18T19:54:22Z
dc.date.available2024-09-18T19:54:22Z
dc.date.issued2010
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe aim of the present study was to determine the effects of different doses of raffinose and methionine on post-thawed semen quality, lipid peroxidation and antioxidant enzyme activities of Angora buck (Capra hircus ancryrensis) sperm following cryopreservation. Ejaculates collected from three Angora bucks were evaluated and pooled at 37 degrees C. Semen samples, which were diluted with a Tris-based extender containing the additives raffinose (2.5, 5, 10 mM) and methionine (2.5, 5, 10 mM) and an extender containing no antioxidants (control), were cooled to 5 degrees C and frozen in 0.25 ml French straws. Frozen straws were thawed individually at 37 degrees C for 20 s in a water bath for evaluation. The freezing extender supplemented with 2.5 and 5 mM methionine led to higher percentages of CASA motility (63.6 +/- 7.0; 63.4 +/- 3.1%, respectively), in comparison to the controls (P < 0.01) following the freeze-thawing process. The addition of antioxidants did not provide any significant effect on the percentages of post-thaw subjective and CASA progressive motilities as well as sperm motion characteristics (VSL and VCL), compared to the control groups (P > 0.05). The freezing extender with raffinose (5 and 10 mM) and methionine at three different doses (2.5, 5 and 10 mM) led to lower percentages of acrosome abnormalities, in comparison to the controls (P < 0.001). In the comet test, raffinose (5 and 10 mM) and methionine (10 mM) gave scores lower than those of the controls, and thereby reduced DNA damage (P < 0.05). Malondialdehyde formation was found to be lower (1.8 +/- 0.1 nmol/L) in the group of 5 mM raffinose, compared to the controls following the freeze-thawing process (P < 0.01). The additives did not show any effectiveness on the maintenance of SOD, GSH-PX and GSH activities, when compared to the controls (P > 0.05). In conclusion, methionine and raffinose play a cryoprotective role against sperm CASA motility, acrosome abnormality and DNA damage. Raffinose 5 mM exhibited antioxidative properties, decreasing MDA levels. Further studies are required to obtain more concrete results on the characterization of microscopic parameters and antioxidant activities in cryopreserved goat sperm with different additives. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipDirectorate of Lalahan Livestock Central Research Institute, Ankara, Turkeyen_US
dc.description.sponsorshipThis study was supported by Directorate of Lalahan Livestock Central Research Institute, Ankara, Turkey.en_US
dc.identifier.doi10.1016/j.cryobiol.2010.05.005
dc.identifier.endpage93en_US
dc.identifier.issn0011-2240
dc.identifier.issn1090-2392
dc.identifier.issue1en_US
dc.identifier.pmid20515679en_US
dc.identifier.scopus2-s2.0-77955430561en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage89en_US
dc.identifier.urihttps://doi.org/10.1016/j.cryobiol.2010.05.005
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7693
dc.identifier.volume61en_US
dc.identifier.wosWOS:000281073900014en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofCryobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAngora buck semenen_US
dc.subjectAntioxidantsen_US
dc.subjectAntioxidant activityen_US
dc.subjectCryopreservationen_US
dc.subjectSperm characteristicsen_US
dc.titleThe effect of raffinose and methionine on frozen/thawed Angora buck (Capra hircus ancryrensis) semen quality, lipid peroxidation and antioxidant enzyme activitiesen_US
dc.typeArticleen_US

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