Characterization of Plum pox potyvirus (PPV) by DAS-ELISA and RT-PCR/RFLP analysis in Turkey

Basit Öğe Kaydı
dc.authoridULUBAS SERCE, CIGDEM/0000-0001-5337-5883
dc.contributor.authorCaglayan, K
dc.contributor.authorSertkaya, G
dc.contributor.authorUlubas, C
dc.contributor.authorKölber, M
dc.contributor.authorKrizbai, L
dc.date.accessioned2024-09-18T20:54:19Z
dc.date.available2024-09-18T20:54:19Z
dc.date.issued2004
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description19th International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops -- JUL 21-25, 2003 -- Anaheim, CAen_US
dc.description.abstractThis study was conducted to determine the presence of Plum pox virus (PPV) (family Potyviridae, genus Potyvirus) in different regions of Turkey and to characterise PPV isolates by serological and molecular techniques including ELISA, PCR/ RFLP. Thus, leaf samples from different stone fruit species, almond, apricot, nectarine, peach, plum, sour and sweet cherry exhibiting various types of symptoms related to PPV were collected from different parts of the canopy from randomly selected orchards in the main stone fruit growing areas in Turkey. Polyclonal antibodies (PAbs) were used to detect the presence of PPV in the plant samples by serological assays (DAS-ELISA). The following monoclonal antibodies (Mabs): Mab5B (Universal), Mab4DG5 (PPV-D: Dideron-specific), MabAL (PPV-M: Marcus-specific), MabEA24 (PPV-El Amar-specific) and MabAC (PPV-C: Cherry-specific) were used to identify the serotyping of PPV isolates. Reverse transcription-polymerase chain reaction (RT-PCR) assays and restriction fragment length polymorphism (RFLP) analysis of RT-PCR products were performed to characterize Turkish PPV isolates. The results of RT-PCR analyses using general primers were in complete agreement with the DAS-ELISA and DASI-ELISA results, showing that 2 apricot samples of 52 stone fruit samples collected from Ankara province were found to be infected with M strain of PPV. This study confirmed the results of the previous work demonstrated the presence of PPV-M strain on apricot in Turkey.en_US
dc.description.sponsorshipInst Valenciano Invest Agrarias,Conseller Agr Pesca Alimentac Generalitat Valenciana,Minist Educ Cienc Direct Gen invest,Univ Politecnica Valencia,Agcy Valenciana Cienc Tecnol,Inst Biol Mole Celular Plantas,Soc Espanola Ciencias Hort,Soc Espanol Fitopatol,Bioreba,Durviz,Loeween_US
dc.identifier.doi10.17660/ActaHortic.2004.657.25
dc.identifier.endpage188en_US
dc.identifier.isbn90-6605-148-5
dc.identifier.issn0567-7572
dc.identifier.issn2406-6168
dc.identifier.issue657en_US
dc.identifier.scopus2-s2.0-58049214197en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage183en_US
dc.identifier.urihttps://doi.org/10.17660/ActaHortic.2004.657.25
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11738
dc.identifier.wosWOS:000226753300025en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInt Soc Horticultural Scienceen_US
dc.relation.ispartofProceedings of The Xixth International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops: Fruit Tree Diseasesen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPlum pox virusen_US
dc.subjectELISAen_US
dc.subjectRT-PCRen_US
dc.subjectRFLP analysisen_US
dc.titleCharacterization of Plum pox potyvirus (PPV) by DAS-ELISA and RT-PCR/RFLP analysis in Turkeyen_US
dc.typeConference Objecten_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu