First molecular characterization of visna/maedi viruses from naturally infected sheep in Turkey

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dc.authoridBertolotti, Luigi/0000-0001-7931-4528
dc.authoridMUZ, Dilek/0000-0001-9358-8103
dc.authoridReina, Ramses/0000-0003-1265-9139
dc.authoridOguzoglu, Tuba Cigdem/0000-0003-4021-2414
dc.authoridRosati, Sergio/0000-0002-8315-0316
dc.contributor.authorMuz, Dilek
dc.contributor.authorOguzoglu, Tuba Cigdem
dc.contributor.authorRosati, Sergio
dc.contributor.authorReina, Ramses
dc.contributor.authorBertolotti, Luigi
dc.contributor.authorBurgu, Ibrahim
dc.date.accessioned2024-09-18T21:02:55Z
dc.date.available2024-09-18T21:02:55Z
dc.date.issued2013
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractRecent worldwide serological and genetic studies of small ruminant lentiviruses (SRLV) have led to the description of new genotypes and the development of new diagnostic tests. This study investigated the detection and molecular characterization of visna/maedi virus (VMV) infection in serum and blood samples from pure and mixed sheep breeds acquired from different regions in Turkey using ELISA and PCR techniques. The prevalence of VMV was 67.8 % by ELISA and/or LTR-PCR with both assays showing a medium level of agreement (kappa: 0.26; +/- A 0.038 CI). Positivity of VMV in sheep increased according to the age of the animal, although PCR positivity was higher than ELISA in young individuals. Phylogenetic analysis of 33 LTR sequences identified two distinct clades that were closely related to American and Greek LTR sequences. Phylogenetic analysis of 10 partial gag gene sequences identified A2, A3, A5, A9, A11 subtypes of genotype A SRLVs. In vitro culture of all isolates in fetal sheep lung cells (FSLC) showed a slow/low phenotype causing less or no lytic infection compared with infection with the WLC-1 American strain characterized by a rapid/highly lytic phenotype. Phylogenetic analysis revealed that Turkish VMV sequences preceded the establishment of American or Greek strains that were associated with the migration of sheep from the Middle East to Western Europe several centuries ago. This is the first study that describes Turkish VMV sequences with the molecular characterization of LTR and gag genes, and it strongly suggests that SRLV-genotype A originated in Turkey.en_US
dc.identifier.doi10.1007/s00705-012-1518-1
dc.identifier.endpage570en_US
dc.identifier.issn0304-8608
dc.identifier.issn1432-8798
dc.identifier.issue3en_US
dc.identifier.pmid23124887en_US
dc.identifier.scopus2-s2.0-84874665417en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage559en_US
dc.identifier.urihttps://doi.org/10.1007/s00705-012-1518-1
dc.identifier.urihttps://hdl.handle.net/20.500.12483/13139
dc.identifier.volume158en_US
dc.identifier.wosWOS:000315561900005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Wienen_US
dc.relation.ispartofArchives of Virologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSmall-Ruminant Lentivirusesen_US
dc.subjectMaedi-Visnaen_US
dc.subjectMixed Flocksen_US
dc.subjectGoatsen_US
dc.subjectTransmissionen_US
dc.subjectSensitivityen_US
dc.subjectSamplesen_US
dc.subjectElisaen_US
dc.subjectMilken_US
dc.subjectPcren_US
dc.titleFirst molecular characterization of visna/maedi viruses from naturally infected sheep in Turkeyen_US
dc.typeArticleen_US

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