Comparison of sperm quality and DNA integrity in mouse sperm exposed to various cooling velocities and osmotic stress

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dc.authoridMcKerlie, Colin/0000-0002-2232-0967
dc.contributor.authorYildiz, Cengiz
dc.contributor.authorLaw, Napoleon
dc.contributor.authorOttaviani, Palma
dc.contributor.authorJarvi, Keith
dc.contributor.authorMcKerlie, Colin
dc.date.accessioned2024-09-18T20:59:04Z
dc.date.available2024-09-18T20:59:04Z
dc.date.issued2010
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe first objective was to compare sperm quality following conventional manual sperm freezing (cryovials held 1, 2, 3, and 4 cm, respectively, above liquid nitrogen (LN2) for 10 min, resulting in cooling velocities of approximately -14.9, -10.1, -6.6, and -5.1 degrees C/min, respectively), and cooling velocities of -5, -20, -40, and -100 degrees C/min in a programmed automated freezer, for sperm recovered from CD-1, B6129SF1, and C57BL/6NCrlBR mice. Furthermore, using these strains, as well as 129S/SvPaslco, and DBA/2NCrlBR mice, the second objective was to determine the effects on DNA integrity of sperm exposed to hyposmotic (1 mOsm/L) and hyperosmotic (2400 mOsm/L) solutions, compared to an isosmotic control (300 mOsm/L). For freezing above LN2 or in an automated freezer, 2 cm above LN2 and -100 degrees C/min, respectively, were optimal (P < 0.05-0.01), with no significant differences between these two approaches for post-thaw progressive motility, DNA integrity, and in vitro rates of fertilization and blastocyst formation. Both manual and automated freezing techniques increased post-thaw sperm DNA fragmentation (P < 0.01); the DNA integrity of post-thaw sperm was significantly affected by cooling velocity and strain background. Relative to isosmotic controls, a hyposmotic solution was more deleterious (P < 0.05-0.01) to sperm DNA integrity than a hyperosmotic solution for CD-1, B6129SF1, C57BL/6, and DBA mice (there were strain-dependent differences). In conclusion, optimization of freezing distance and cooling velocity (manual and automated freezing, respectively) were significant factors for efficient cryopreservation and re-derivation of mice from frozen-thawed sperm. Additionally, osmotically-driven volume changes in mouse sperm increased DNA fragmentation, with susceptibility affected by background strain. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipGenome Canada through the Ontario Genomics Institute and Genome Prairie; Canadian Institutes of Health Research [80152]; Canada Foundation for Innovation; Ontario Innovation Trust; Charles River Canada; Ontario Ministry of Innovation; Academy of Finland (AKA) [80152] Funding Source: Academy of Finland (AKA)en_US
dc.description.sponsorshipThis research was supported by Genome Canada through the Ontario Genomics Institute and Genome Prairie; Canadian Institutes of Health Research grants to CM (FRN #80152); Canada Foundation for Innovation and Ontario Innovation Trust grants to CM; and Charles River Canada. CM is a recipient of the Ontario Ministry of Innovation's Early Researcher Award.en_US
dc.identifier.doi10.1016/j.theriogenology.2010.06.014
dc.identifier.endpage1430en_US
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.issue8en_US
dc.identifier.pmid20728931en_US
dc.identifier.scopus2-s2.0-77957293243en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1420en_US
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2010.06.014
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12345
dc.identifier.volume74en_US
dc.identifier.wosWOS:000283265000014en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTheriogenologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSpermen_US
dc.subjectEmbryoen_US
dc.subjectCryopreservationen_US
dc.subjectDNA integrityen_US
dc.subjectIn vitro fertilizationen_US
dc.subjectMouseen_US
dc.titleComparison of sperm quality and DNA integrity in mouse sperm exposed to various cooling velocities and osmotic stressen_US
dc.typeArticleen_US

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