Detection of Coxiella burnetii in cows', goats', and ewes' bulk milk samples using polymerase chain reaction (PCR)

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dc.contributor.authorCan, Hayriye Yesim
dc.contributor.authorElmali, Mehmet
dc.contributor.authorKaragoz, Alper
dc.date.accessioned2024-09-18T20:06:17Z
dc.date.available2024-09-18T20:06:17Z
dc.date.issued2015
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractQ fever is a widespread zoonotic disease that is caused by obligate intracellular bacteria, Coxiella burnetii. This study was planned to determine the prevalence of C. burnetii in cows', goats', and ewes' bulk milk (BM) samples using PCR and to confirm positive results by DNA sequencing. A total of 150 BM samples (50 samples of each cows', goats', and ewes' milk) collected from 15 randomly selected dairy farms in Hatay province were analyzed. The BM samples were taken between January 2012 and July 2013. Bacterial DNA was extracted directly from milk samples. Nine of the BM samples (6 %) were PCR positive; five from cows' BM, two from ewes' BM, and two from goats' BM. Bacterial DNA was detected in 3 of 15 (20 %) dairy farms. Positive results were confirmed by DNA sequencing. The sequencing results of Coxiella DNA extracted from cows', goats', and ewes' milk samples were consistent with the reference strain with 100 %, 99.7 %, 99.8 % homology, respectively. BM samples were found to be contaminated with C. burnetii; therefore, taking hygienic measures is necessary for food safety and public health.en_US
dc.description.sponsorshipMustafa Kemal University [BAP-1201M0113]en_US
dc.description.sponsorshipThe present study was supported by Mustafa Kemal University, Project code of BAP-1201M0113.en_US
dc.identifier.doi10.15567/mljekarstvo.2015.0104
dc.identifier.endpage31en_US
dc.identifier.issn0026-704X
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84921979941en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage26en_US
dc.identifier.urihttps://doi.org/10.15567/mljekarstvo.2015.0104
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8436
dc.identifier.volume65en_US
dc.identifier.wosWOS:000351252600004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherCroatian Dairy Unionen_US
dc.relation.ispartofMljekarstvoen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectbulk milken_US
dc.subjectCoxiellaen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectsequence analysisen_US
dc.titleDetection of Coxiella burnetii in cows', goats', and ewes' bulk milk samples using polymerase chain reaction (PCR)en_US
dc.typeArticleen_US

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