Molecular Detection and Characterization of 'Candidatus Phytoplasma mali' Associated With Phyllody on Narcissus tazetta in Türkiye

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dc.authoridBoztas, Kadir/0000-0002-4638-4973
dc.contributor.authorBoztas, Kadir
dc.contributor.authorDerecik, Kayhan
dc.contributor.authorCaglayan, Kadriye
dc.contributor.authorTulum, Isil
dc.date.accessioned2024-09-18T20:14:59Z
dc.date.available2024-09-18T20:14:59Z
dc.date.issued2024
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn the winter of 2019, typical phyllody, flower sterility, and stunting symptoms were observed on daffodil plants (Narcissus tazetta) in the Mersin province of T & uuml;rkiye. Molecular analyses were carried out on 16S rRNA genes of these plants to identify the cause of these symptoms and detect the presence of phytoplasma. DNA extracted from symptomatic plant samples was amplified via nested polymerase chain reaction using the universal primers P1/P7 followed by R16F2n/R16R2. The 16S rRNA gene amplicons from nine samples which were exhibiting severe phyllody symptoms were sequenced, nucleotide BLAST analysis revealed a 99.9% identity match among them and 99.91% with 'Candidatus Phytoplasma mali'. Further analyses of the 16S rRNA gene-based phylogenetic tree, computer-simulated restriction fragment length polymorphism (virtual RFLP) and conventional RFLP analyses of 16S rRNA gene using MseI, TaqI, RsaI and SspI restriction endonucleases led to assign the phytoplasma to 16Sr group X and subgroup A. For finer characterization of 'Ca. P. mali' strain in daffodils, a PCR based multilocus sequence analysis (MLSA) was performed on three distinct genetic loci including nitroreductase and rhodanase-like genes, ribosomal protein genes rpl22 and rps3, and secY gene. The BLAST results showed 99.9% match to 'Ca. P. mali' AP-15 subtype (GenBank Accession Number: AJ542541). Electron microscopic examination of the infected daffodil plants revealed the presence of pleomorphic phytoplasma units in the sieve elements of the phloem tissues. Although 16SrX-A subgroup phytoplasmas have frequently been reported from woody plants, to our knowledge this is the first report of 'Ca. P. mali' (16SrX-A) infecting daffodil plants worldwide.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey to I.T. under the TUBITAK-2232 Fellowship for Outstanding Researchers [:118C290]; Istanbul University Scientific Research Projects Administration (BAP) [34962, 36648]en_US
dc.description.sponsorshipThis work was funded by the Scientific and Technological Research Council of Turkey to I.T. under the TUBITAK-2232 Fellowship for Outstanding Researchers (no:118C290) and Istanbul University Scientific Research Projects Administration (BAP) grants (no: 34962) to K.B. and (no: 36648) to K.D.en_US
dc.identifier.doi10.1007/s10343-024-00982-0
dc.identifier.endpage691en_US
dc.identifier.issn2948-264X
dc.identifier.issn2948-2658
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85202033243en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage683en_US
dc.identifier.urihttps://doi.org/10.1007/s10343-024-00982-0
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9360
dc.identifier.volume76en_US
dc.identifier.wosWOS:001206251700002en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Crop Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDaffodilen_US
dc.subjectPhyllodyen_US
dc.subjectPhytoplasmaen_US
dc.subjectPCR/RFLPen_US
dc.subjectMultilocus gene analysesen_US
dc.subjectTEMen_US
dc.titleMolecular Detection and Characterization of 'Candidatus Phytoplasma mali' Associated With Phyllody on Narcissus tazetta in Türkiyeen_US
dc.typeArticleen_US

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