Development of pmp Gene-Specific PCR Assay with A Host Specific Internal Control for Chlamydophila felis

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dc.authoridaltug, nuri/0000-0001-5805-0340
dc.contributor.authorCantekin, Zafer
dc.contributor.authorSolmaz, Hasan
dc.contributor.authorAltug, Nuri
dc.contributor.authorOzmen, Gamze Ozge
dc.date.accessioned2024-09-18T20:08:04Z
dc.date.available2024-09-18T20:08:04Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Ãœniversitesien_US
dc.description.abstractChlamydophila felis is the primary bacterial agent of conjunctivitis and upper respiratory disease in cats. Transmission of the disease requires close contact between cats. Polymerase chain reaction is a useful tool for detection of this organism. The aim of this study was to develop a polymerase chain reaction assay with an internal amplification control for the detection of C. felis. Primer pairs were designed specifically for polymorphic membrane protein gene of C. felis and cytochrome b gene of cat, and their specificity and sensitivity were examined. Primers specific for both genes were then multiplexed. In the simplex polymerase chain reaction analyses with 10-fold dilutions, C. felis DNA was detected with designed primers for polymorphic membrane protein genes up to 1.6 pg/mu l and cat DNA was demonstrated in all samples in the polymerase chain reaction. Moreover, in the multiplex polymerase chain reaction, C. felis DNA and cat DNA were detected together. These designed primers specific for C. felis might have potential for research on infections and shedding of this organism in cats as the internal control host specific primers might have potential for using internal control for detection of different microorganisms in cats.en_US
dc.identifier.endpage476en_US
dc.identifier.issn0125-6491
dc.identifier.issue4en_US
dc.identifier.startpage469en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8604
dc.identifier.volume44en_US
dc.identifier.wosWOS:000346948500008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherChulalongkorn Univen_US
dc.relation.ispartofThai Journal of Veterinary Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCaten_US
dc.subjectChlamydophila felisen_US
dc.subjectmultiplex polymerase chain reactionen_US
dc.subjectpolymorphic membrane protein geneen_US
dc.titleDevelopment of pmp Gene-Specific PCR Assay with A Host Specific Internal Control for Chlamydophila felisen_US
dc.typeArticleen_US

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