Leishmaniasis in Turkey: Determination of Leishmania species by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)

Basit Öğe Kaydı
dc.authorscopusid6506955731
dc.authorscopusid26038972000
dc.authorscopusid7801422478
dc.authorscopusid6602818486
dc.authorscopusid6603599022
dc.authorscopusid34768598400
dc.authorscopusid22980624200
dc.contributor.authorCulha, Gülnaz
dc.contributor.authorAkyar, Isin
dc.contributor.authorYildiz Zeyrek, Fadile
dc.contributor.authorKurt, Özgür
dc.contributor.authorGündüz, Cumhur
dc.contributor.authorÖzensoy Töz, Seray
dc.contributor.authorÖstan, Ipek
dc.date.accessioned2024-09-19T15:46:59Z
dc.date.available2024-09-19T15:46:59Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground: Cutaneous leishmaniasis (CL) is endemic in Southeastern Anatolia, mainly in Sanliurfa and Hatay provinces, and the causative agents are mostly Leishmania tropica and less frequently L. infantum. Here, we report the first MALDI-TOF analyses of Leishmania promastigotes obtained from the cultures of two CL cases from Osmaniye and Hatay provinces who were initially diagnosed by microscopy, culture and identified as L. infantum with Real-Time PCR (RT-PCR). Methods: Samples obtained from the skin lesions of patients were initially stained with Giemsa and cultivated in NNN medium. Examination of the smears and cultures revealed Leishmania amastigotes and promastigotes, respectively. The promastigotes (MHOM/TR/2012/CBU15 and MHOM/TR/2012/MK05) obtained from the cultures of both patients were used for RT-PCR targeting the ITS-1 region in the SSU of rRNA. The reference strains of four Leishmania species (L. infantum, L. donovani, L. tropica and L. major) were initially assessed with MALDI-TOF and their data were added to MALDI-TOF Biotyper Library. Results: Both RT-PCR and MALDI-TOF analyses indicated that the causative agent in both patient samples was L. infantum. Conclusion: Despite disadvantages such as requirement of culture fluid with nothing but promastigotes and high cost, MALDI-TOF analysis may be a fast, sensitive and specific diagnostic tool in especially large-scale research studies, where the cost declines, relatively.en_US
dc.identifier.endpage248en_US
dc.identifier.issn1735-7020
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84902468258en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage239en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14890
dc.identifier.volume9en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTehran University of Medical Sciences (TUMS)en_US
dc.relation.ispartofIranian Journal of Parasitologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCutaneous leishmaniasisen_US
dc.subjectLeishmania infantumen_US
dc.subjectMALDI -TOFen_US
dc.subjectReal-Time PCRen_US
dc.subjectTurkeyen_US
dc.titleLeishmaniasis in Turkey: Determination of Leishmania species by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)en_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu