Determination of expression patterns of miR-26a, and preimplantation factor levels for early pregnancy detection in nulliparous and multiparous cows

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dc.authoridOzkan, Huseyin/0000-0001-5753-8985
dc.authoridKaya, Ufuk/0000-0002-4805-0993
dc.authoridOLGAC, KEMAL TUNA/0000-0001-9216-7059
dc.contributor.authorOlgac, Kemal Tuna
dc.contributor.authorYazlik, Murat Onur
dc.contributor.authorOzkan, Huseyin
dc.contributor.authorKaya, Ufuk
dc.contributor.authorTirpan, Mehmet Borga
dc.date.accessioned2024-09-18T20:13:29Z
dc.date.available2024-09-18T20:13:29Z
dc.date.issued2024
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractFor maximum productivity in a dairy farm, the earliest and the most accurate detection of pregnancy is essential. The aim of this study was to determine the efficacy of expression patterns of miR-26a, and serum Preimplantation Factor (PIF) levels for pregnancy diagnosis during the early pregnancy in nulliparous and multiparous cows. A total of 60 cows (30 nulliparous and 30 multiparous Holstein cows) were enrolled in the study. Blood samples were collected for miR-26a on days 8 and 16 (D8 and D16), and for the PIF on days 10 and 20 (D10 and D20) following insemination (D0). Pregnancies were determined by ultrasonography on the 28th day after insemination. Expression levels of miR-26a determined by qPCR. PIF levels were assessed by using commercial ELISA kits. All data were analyzed by using the MIXED procedure of SPSS. The expression levels of miR-26a were 6.64 folds higher on D16 in pregnant compared to non-pregnant multiparous cows (p < .05). On D8 and D16, miR-26a expression levels were found higher 13 folds in pregnant compared to non-pregnant nulliparous cows (p < .05). Additionally, miR-26a expressions were higher 5.42 folds (p < .05) on D8, 7.19 folds higher (p < .01) on D16 in pregnant nulliparous and multiparous cows, and were 6.30 folds higher (p < .001) on D8 and D16 according to non-pregnant animals. PIF levels were greater in pregnant animals (p < .05). Analyzing miR-26a on D8 might be considered as sufficient in nulliparous cows. Pregnancy detection in multiparous cows can be made on the 16th day with this method. Furthermore, PIF evaluations may be sufficient on D10 in multiparous cows. Besides, PIF levels and miR-26a expression levels might be used safely in field conditions and clinical applications.en_US
dc.description.sponsorshipTrkiye Bilimsel ve Teknolojik Arascedil;timath;rma Kurumu [221O318]; Scientific and Technological Research Council of Turkiye (TUBITAK)en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkiye (TUBITAK) (Project number: 221O318), Turkiye.en_US
dc.identifier.doi10.1111/rda.14521
dc.identifier.issn0936-6768
dc.identifier.issn1439-0531
dc.identifier.issue1en_US
dc.identifier.pmid38268207en_US
dc.identifier.scopus2-s2.0-85181190675en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1111/rda.14521
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9217
dc.identifier.volume59en_US
dc.identifier.wosWOS:001131495700001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofReproduction in Domestic Animalsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcowsen_US
dc.subjectearly pregnancyen_US
dc.subjectmiR-26aen_US
dc.subjectmultiparousen_US
dc.subjectnulliparousen_US
dc.subjectpreimplantation factor proteinen_US
dc.titleDetermination of expression patterns of miR-26a, and preimplantation factor levels for early pregnancy detection in nulliparous and multiparous cowsen_US
dc.typeArticleen_US

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