Detection of some viruses of stone fruits in mother plant blocks in eastern Mediterranean region of Turkey
[ N/A ]
Tarih
2004
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Int Soc Horticultural Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Field inspection and sample collection were carried out in mother plant blocks which are also varietal collection belonging to the Ministry of Agriculture and the Universities in Eastern Mediterranean Region of Turkey during early spring and in autumn in 2002. Samples (shoots and leaves) were collected from symptomatic plants in the orchards. 48 samples (3 almond, 9 apricot, 3 nectarine, 10 peach, It plum, 12 sweet cherry) having different symptoms were collected and tested for the presence of Apple chlorotic leaf spot trichovirus (ACLSV), Apple mosaic ilarvirus (ApMV), Cherry leaf roll nepovirus (CLRV), Prune dwarf ilarvirus (PDV), Prunus necrotic ringspot ilarvirus (PNRSV) and Plum pox potyvirus (PPV) by using DAS-ELISA. According to serological assays, the most common virus was PDV. It was followed by PNRSV and ACLSV. The reverse transcription-polymerase chain reaction (RT-PCR) assays were performed for investigation of PDV, PNRSV and PPV in the samples. The following viruses were identified by ELISA and RT-PCR assays: ACLSV (1 sample), CLRV (1 sample), PDV (5 samples), PNRSV (2 samples). Mix infections of ACLSV+PDV (1 sample) and PDV+PNRSV (2 samples) were also found. No PPV-infected samples were detected from the samples collected from mother plant blocks in Eastern Mediterranean Region of Turkey.
Açıklama
19th International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops -- JUL 21-25, 2003 -- Anaheim, CA
Anahtar Kelimeler
DAS-ELISA, mother plant blocks, RT-PCR, stone fruit viruses
Kaynak
Proceedings of The Xixth International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops: Fruit Tree Diseases
WoS Q Değeri
N/A
Scopus Q Değeri
Q4
Cilt
Sayı
657
