Prevalence and Characterization of ESBL- and AmpC-producing Escherichia coli from Cattle

Basit Öğe Kaydı
dc.contributor.authorAslantas, Ozkan
dc.contributor.authorElmacioglu, Sibel
dc.contributor.authorYilmaz, Ebru Sebnem
dc.date.accessioned2024-09-18T20:19:51Z
dc.date.available2024-09-18T20:19:51Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn this study, it was aimed to determine the prevalence of extended spectrum beta-lactamase (ESBL) and/or AmpC type beta-lactamase (AmpC) producing Escherichia coli from cattle in Hatay. For this purpose, 312 rectal swabs samples were collected from apparently healthy cattle. ESBL production was phenotypically investigated by disc combination method and double disc synergism test and beta-lactamase genes (bla(CTX-M), bla(CMY-2), bla(SHV), bla(OXA), and bla(TEM)) and plasmid mediated quinolone resistance (PMQR) genes (qnrA, qnrB, qnrS and aac(6')-Ib) were screened by polymerase chain reaction (PCR) and subsequent sequence analysis. Antimicrobial susceptibility of the isolates were determined using disc diffusion method and their phylogenetic groups were also searched by PCR. Twenty six (8.3%) isolates were found to be ESBL producer by phenotypic tests. The following ESBL/AmpC genes were detected: bla(CTX-M-15) (n=12), bla(CTX-M-1) (n=11), bla(CTX-M-3) (n=2), and bla(CMY-2) (n=1). PMQR genes were detected in 11 (42.3%) ESBL producing E. coli isolates and these isolates were only positive for aac(6')-Ib-cr and qnrS1 genes. Twenty two (84.6%) of the isolates exhibited multidrug resistance (MDR) phenotype. ESBL/AmpC producing E. coli isolates were observed to be belonged to B1 (50%), A (34.6%) and D (15.4%) phylogroups. This study was the first to describe the presence of CTX-M-15, CTX-M-3, CTX-M-1 and CMY-2 producing E. coli in cattle in Turkey and the co-existence of aac(6')-Ib-cr and qnrS1 genes in some isolates.en_US
dc.description.sponsorshipMustafa Kemal University Scientific Research Fund [8440]en_US
dc.description.sponsorshipThis work was supported by Mustafa Kemal University Scientific Research Fund (Project Number: 8440)en_US
dc.identifier.doi10.9775/kvfd.2016.15832
dc.identifier.endpage67en_US
dc.identifier.issn1300-6045
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84992449510en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage63en_US
dc.identifier.urihttps://doi.org/10.9775/kvfd.2016.15832
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9902
dc.identifier.volume23en_US
dc.identifier.wosWOS:000388115200009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEscherichia colien_US
dc.subjectCattleen_US
dc.subjectESBLen_US
dc.subjectAmpCen_US
dc.titlePrevalence and Characterization of ESBL- and AmpC-producing Escherichia coli from Cattleen_US
dc.typeArticleen_US

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