The effects of increased cAMP content on inflammation, oxidative stress and PDE4 transcripts during Brucella melitensis infection

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dc.contributor.authorErdogan, Suat
dc.contributor.authorAslantas, Ozkan
dc.contributor.authorCelik, Sefa
dc.contributor.authorAtik, Esin
dc.date.accessioned2024-09-18T20:06:15Z
dc.date.available2024-09-18T20:06:15Z
dc.date.issued2008
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractCyclic AMP (cAMP) is a key intracellular second messenger which at increased levels has been shown to have anti-inflammatory and tissue-protective effects. Its concentration is determined by the activities of both adenylate cyclase (AC) and the phosphodiesterase (PDE) enzymes. The aim of this study was to compare the effects of increased cAMP and glucocorticoid dexamethasone administration on B. melitensis-induced lipid peroxidation, Brucella suppressed antioxidant enzyme activities and PDE4 transcripts in rats. Intracellular cyclic AMP level was elevated by two different approaches; activation of AC and inhibition of PDE activities. Rats were inoculated with B. melitensis for seven days then a single dose of nonselective PDE inhibitor 3-isobutyl-1-methylxanthine.(IBMX), the adenylate cyclase activator forskolin and dexamethasone were administrated to each infected group, and animals were challenged for 48 h. Brucella-induced lipid peroxidation was significantly reduced by the cAMP elevating agents as well as dexamethasone administration in plasma, liver and spleen. The antioxidant enzymes glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were significantly decreased by the pathogen. Whilst suppressed GSH-Px activity was reversed by cAMP elevating agents, SOD activity was not restored. Superoxide generating enzyme xanthine oxidase activity was not altered at the end of the infection period. Brucella infection increased plasma IL-12 level and this effect was also suppressed by the cAMP elevating agents, whereas TNF-alpha, IFN-gamma and IL-10 levels were unchanged. Intracellular cAMP levels are entirely hydrolyzed by cAMP-specific PDE 4 isozymes (PDE4s) in inflammatory and immunocompetent cells. Brucella reduced mRNA transcript levels for PDE4A by 40%, though PDE4B and 4D transcriptions were being unaffected in spleen. It was concluded that B. melitensis infection decreased activity of the antioxidant defence system, induced lipid peroxidation and suppressed PDE4A transcription. Administration of cAMP elevating agents exhibited similar affect with dexamethasone on lipid peroxidation, IL-12 production and antioxidant enzyme activities in Brucella infection. (C) 2007 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.rvsc.2007.02.003
dc.identifier.endpage25en_US
dc.identifier.issn0034-5288
dc.identifier.issn1532-2661
dc.identifier.issue1en_US
dc.identifier.pmid17397885en_US
dc.identifier.scopus2-s2.0-36049050697en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage18en_US
dc.identifier.urihttps://doi.org/10.1016/j.rvsc.2007.02.003
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8415
dc.identifier.volume84en_US
dc.identifier.wosWOS:000251935000004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofResearch in Veterinary Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBrucella melitensisen_US
dc.subjectcytokineen_US
dc.subjectforskolinen_US
dc.subjectIBMXen_US
dc.subjectlipid peroxidationen_US
dc.subjectoxidative stressen_US
dc.subjectPDE4en_US
dc.subjectphosphodiesteraseen_US
dc.titleThe effects of increased cAMP content on inflammation, oxidative stress and PDE4 transcripts during Brucella melitensis infectionen_US
dc.typeArticleen_US

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