Detection of bovine papillomavirus in an equine cell line as a contaminant

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dc.authoridOguzoglu, Tuba Cigdem/0000-0003-4021-2414
dc.authoridAkkutay-Yoldar, Zeynep/0000-0002-1178-5347
dc.contributor.authorAkkutay-Yoldar, Z.
dc.contributor.authorOguzoglu, T.
dc.contributor.authorDogan, F.
dc.contributor.authorAtaseven, V
dc.date.accessioned2024-09-18T21:06:36Z
dc.date.available2024-09-18T21:06:36Z
dc.date.issued2019
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBovine papillomaviruses (BPV) are members of the family Papillomaviridae. Papillomaviruses are usually species-specific and epitheliotropic. Delta-BPVs are an exception to this rule in that they can also infect fibroblasts and non-bovid ungulates. Cell cultures are essential for performing in vitro studies, analysis of virus biology, vaccine production, tissue engineering and toxicity testing. In this context, cell line contamination constitutes a significant problem. In this study, various cell lines (n=27) were assessed for potential BPV contamination. To this aim, DNA was extracted from cell cultures and then screened for the presence of papillomavirus L1 capsid gene DNA using a consensus polymerase chain reaction (PCR) system. Immunofluorescence (IF) staining was used for viral protein detection. Intriguingly, one cell line derived from equine dermis tested positive by PCR and subsequent IF staining for L1. Amplicon sequencing followed by computed L1 DNA sequence alignment led to the identification of a new putative BPV type, revealing the highest identities with Delta-BPV types 1 (76%) and 2 (73%). To the authors' knowledge, this is the first report on the presence of a putative BPV as a viral contaminant in cell cultures that possibly represents an unknown Delta-BPV.en_US
dc.identifier.doi10.21825/vdt.v88i3.16019
dc.identifier.endpage156en_US
dc.identifier.issn0303-9021
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85072778949en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage150en_US
dc.identifier.urihttps://doi.org/10.21825/vdt.v88i3.16019
dc.identifier.urihttps://hdl.handle.net/20.500.12483/13717
dc.identifier.volume88en_US
dc.identifier.wosWOS:000472893700003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUniv Ghenten_US
dc.relation.ispartofVlaams Diergeneeskundig Tijdschriften_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCutaneous Tumorsen_US
dc.subjectDnaen_US
dc.subjectClassificationen_US
dc.subjectCulturesen_US
dc.subjectSarcoidsen_US
dc.subjectFelineen_US
dc.subjectType-1en_US
dc.titleDetection of bovine papillomavirus in an equine cell line as a contaminanten_US
dc.typeArticleen_US

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