Pathological and Microbiological Investigations of Pneumonic Pasteurellosis in Sheep
Yükleniyor...
Dosyalar
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kafkas Univ, Veteriner Fakultesi Dergisi
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, between March 2010 and March 2011, 110 pneumonia suspected lung tissues were examined histopathologically, immunohistochemically and microbiologically, in Sanliurfa province. After definition of the macroscopic localization of the consolidated areas in the lungs, tissue samples were taken and preserved in cold chain and 10% formalin for microbiological and pathological examinations, respectively. For bacteriological examination of Pasteurella spp. lung specimens were inoculated in 7% sheep blood agar and Mc Conkey agar. After routine pathological tissue follow up procedure, tissues were embedded in paraffin and obtained sections stained with Hematoxylin&Eosin (H&E). The cases, having histopathological findings consistent with pneumonia, were forwarded to immunohistochemical (IHC) examinations to know whether lesions related to Mannheimia haemolytica and Pasteurella multocida using hyperimmune polyclonal rabbit sera in Avidin Biotin Complex Peroxidase (ABC-P). Microbiological, histopathological and immunohistochemical findings were comparatively evaluated in examined animals. Pasteurella multocida as a cause of pneumonia were detected in 38 cases of microbiological inoculations. Immunohistochemical staining resulted Mannheimia haemolytica (n=35) and Pasteurella multocida (n=30) positive. Immunohistochemically both Mannheimia haemolytica and Pasteurella multocida were positive in 23 cases and 45 animals were negative for both bacteria. The aim of this study is to show importance and role of Pasteurella spp, in sheep pneumonia in Sanliurfa region.
Açıklama
Anahtar Kelimeler
Pneumonic Pasteurellosis, Pathology, Microbiology, Immunohistochemistry, Sheep
Kaynak
Kafkas Universitesi Veteriner Fakultesi Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
19
Sayı
1
