Agrobacterium-mediated transformation of 'Kirkagac 637' a recalcitrant melon (Cucumis melo L.) cultivar with ZYMV coat protein encoding gene

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dc.authoridipek, muzaffer/0000-0002-5773-7236
dc.authoridGaba, Victor/0000-0001-5405-9946
dc.contributor.authorYalcin-Mendi, NY
dc.contributor.authorIpek, M
dc.contributor.authorSerbest-Kobaner, S
dc.contributor.authorCuruk, S
dc.contributor.authorKacar, YA
dc.contributor.authorCetiner, S
dc.contributor.authorGaba, V
dc.date.accessioned2024-09-18T20:29:50Z
dc.date.available2024-09-18T20:29:50Z
dc.date.issued2004
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractA gene encoding for zucchini yellow mosaic virus (ZYMV) coat protein was transformed into 'Kirkagac 637', an important Turkish melon cultivar using Agrobacterium tumefaciens, and putatively resistant transgenic fines were obtained. Proximal cotyledonary explants were incubated with Agrobacterium carrying the binary plasmid PGA643 including the nptii and zymv coat protein genes under the control of the CaMV 35S promoter. ELISA tests and PCR analyses were performed to determine which plants were transformants. Transformation efficiency was 1,6 %. Four transformants were self pollinated to obtain R-1 generation. The plants from the R-2 generation were tested by PCR to demonstrate the integration of the zymv coat protein gene (1000 bp) in to the 'Kirkagac 637' genome. Segregation of 4 transformants was tested by X-2 analysis and the results were consistent with predicted Mendelian ratios. In each case the P values were much greater than the rejection level of P=0.05.en_US
dc.identifier.endpage262en_US
dc.identifier.issn1611-4426
dc.identifier.issn1611-4434
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-13844257144en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage258en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11085
dc.identifier.volume69en_US
dc.identifier.wosWOS:000226796000006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInt Soc Horticultural Science-Ishsen_US
dc.relation.ispartofEuropean Journal of Horticultural Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmelonen_US
dc.subjectAgrobacterium tumefaciensen_US
dc.subjectZYMV CP geneen_US
dc.subjectregenerationen_US
dc.subjecttransformationen_US
dc.titleAgrobacterium-mediated transformation of 'Kirkagac 637' a recalcitrant melon (Cucumis melo L.) cultivar with ZYMV coat protein encoding geneen_US
dc.typeArticleen_US

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