Prevalence of equine herpesvirus-1 and equine herpesvirus-4 infections in equidae species in Turkey as determined by ELISA and multiplex nested PCR

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dc.authoridTAN, M. Tolga/0000-0001-5253-4010
dc.contributor.authorAtaseven, Veysel S.
dc.contributor.authorBilge Dagalp, Seval
dc.contributor.authorGuzel, Murat
dc.contributor.authorBasaran, Zeynep
dc.contributor.authorTan, Mehmet T.
dc.contributor.authorGeraghty, Bob
dc.date.accessioned2024-09-18T19:54:30Z
dc.date.available2024-09-18T19:54:30Z
dc.date.issued2009
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn this report we examined the presence of specific antibodies against equine herpesvirus type 1 (EHV-1 and equine herpesvirus type 4 (EHV-4) in several equidae, including mules, donkeys, horses. The presence of EHV-1 and EHV-4 in respiratory diseases of equids, and ability of multiplex nested polymerase chain reaction (PCR) screening in simultaneous diagnosis of horses acutely infected by EHV-1 and EHV-4 were also investigated. Sera from 504 horses, mules and donkeys sampled were tested for the presence of EHV-1 and EHV-4 specific antibodies. Blood samples taken from 21 symptomatic horses and nasal swabs taken from 40 symptomatic horses were tested for the presence of EHV-1 and EHV-4 by a multiplex nested PCR. A total of 14.3% (3/21) of buffy coat samples and 32.5% (13/40) nasal swab samples were found to contain EHV-1 DNA, while 19% (4/21) buffy coat samples and 22.5% (9/40) nasal swab samples were found to be positive for EHV-4 DNA. By species, 14.5% of horses, 37.2% of mules and 24.2% of donkeys tested were EHV-1 seropositive. EHV-4 specific antibodies were detected in 237 (81.7%) of 290 horse sera tested. Results from this investigation demonstrate that EHV-1 and EHV-4 are prevalent throughout the equid population, and that donkeys and mules might also represent an important source of infection for other equids. We also showed that the multiplex nested PCR assay might be useful for diagnosis of mixed respiratory infections in horses due to EHV-1 and EHV-4. (C) 2008 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipScientific Research Funds of Mustafa Kemal University [06 G 0501]en_US
dc.description.sponsorshipThe authors wish to thank Dr. Orhan Sahin from College of Veterinary Medicine, University of Iowa State for his critiques and support on the manuscript. A part of this project (Acceptation No. 06 G 0501) was supported by the Scientific Research Funds of Mustafa Kemal University. We also thank Ar-En Medical, for kindly providing some of the ELISA kits used in this study.en_US
dc.identifier.doi10.1016/j.rvsc.2008.06.001
dc.identifier.endpage344en_US
dc.identifier.issn0034-5288
dc.identifier.issn1532-2661
dc.identifier.issue2en_US
dc.identifier.pmid18649902en_US
dc.identifier.scopus2-s2.0-60249094128en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage339en_US
dc.identifier.urihttps://doi.org/10.1016/j.rvsc.2008.06.001
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7768
dc.identifier.volume86en_US
dc.identifier.wosWOS:000264244800026en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofResearch in Veterinary Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntibodyen_US
dc.subjectEquidaeen_US
dc.subjectHerpesvirusen_US
dc.subjectMultiplex nested PCRen_US
dc.subjectRespiratory diseaseen_US
dc.titlePrevalence of equine herpesvirus-1 and equine herpesvirus-4 infections in equidae species in Turkey as determined by ELISA and multiplex nested PCRen_US
dc.typeArticleen_US

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