Effect of Glycerol on Fertility of Cryopreserved Grass Carp (Ctenopharyngodon idella) Sperm
dc.contributor.author | Bozkurt, Yusuf | |
dc.contributor.author | Yavas, Ilker | |
dc.contributor.author | Ogretmen, Fatih | |
dc.contributor.author | Sivasligil, Bugra | |
dc.contributor.author | Karaca, Fikret | |
dc.date.accessioned | 2024-09-18T20:25:30Z | |
dc.date.available | 2024-09-18T20:25:30Z | |
dc.date.issued | 2011 | |
dc.department | Hatay Mustafa Kemal Üniversitesi | en_US |
dc.description.abstract | This research investigated the effect of adding glycerol to different ionic extenders on motility and fertilizing ability of frozen-thawed sperm of grass carp (Ctenopharyngodon idella) under hatchery conditions. Semen was collected by abdominal stripping from adult males and diluted to a ratio of 1:3 in Kurokura I or Kurokura II extender containing 10%, 15%, or 20% glycerol. Diluted semen was packaged in 0.5-ml straws and left to equilibrate for 30 min at 4 degrees C. Following equilibration, the straws were exposed to liquid nitrogen vapor for 10 min and plunged into liquid nitrogen (-196 degrees C). Straws were thawed in a water bath at 30 degrees C for 10 s to determine the post-thaw motility and movement duration. The spermatozoa: egg ratio in fertilization experiments was 1 x 10(5):1. The highest mean fertilization rate (95.63 +/- 0.52; p>0.05) was obtained with semen frozen in Kurokura I extender containing 10% glycerol. Results indicate that grass carp sperm can be successfully cryopreserved with ionic extenders containing glycerol at 10-20%. | en_US |
dc.identifier.issn | 0792-156X | |
dc.identifier.scopus | 2-s2.0-85013329161 | en_US |
dc.identifier.scopusquality | Q4 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12483/10336 | |
dc.identifier.volume | 63 | en_US |
dc.identifier.wos | WOS:000298952500001 | en_US |
dc.identifier.wosquality | Q3 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.language.iso | en | en_US |
dc.publisher | Israeli Journal Of Aquaculture-Bamidgeh | en_US |
dc.relation.ispartof | Israeli Journal of Aquaculture-Bamidgeh | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | grass carp | en_US |
dc.subject | Ctenopharyngodon idella | en_US |
dc.subject | cryopreservation | en_US |
dc.subject | glycerol | en_US |
dc.subject | extender | en_US |
dc.subject | semen | en_US |
dc.subject | fertilization | en_US |
dc.title | Effect of Glycerol on Fertility of Cryopreserved Grass Carp (Ctenopharyngodon idella) Sperm | en_US |
dc.type | Article | en_US |