In vitro antiviral effect of the essential oils of thymbra spicata L. on herpes simplex virus type 2
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Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The essential oils of Thymbra spicata L. has been used for a long time as a folk medicine to treat a lot of diseases. In this study, we aimed to investigate the antiviral effect of The essential oils of Thymbra spicata L. against HSV-2 (Herpes Simplex Virus type 2). Aerial parts of Thymbra spicata L. were used. They harvested at full flowering stage in July 2011 from the botanical gardens, Field Crops Department of Mustafa Kemal University. Chemical analysis of Thymbra spicata L. was performed by GC-MS. In the study, HEp-2 cell line derived from human larynx cancer cells was used. Cultivation of HEp-2 cells was carried out in RPMI 1640 medium with 10% fetal calf serum at an atmosphere of 37°C with 5% CO2. The cell viability was evaluated by a tetrazolium-based colorimetric method using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The main components with the highest rates were carvacrol, cymol, gamaterpinene and thymol. While, typical cytopathological changes for HSV-2 on the cells was observed at low Thymbra spicata L., concentrations (10 and 20 ?g/ml), no cytopathological changes was observed at high Thymbra spicata L., concentrations (40, 80 and 160 ?g/ml). In these concentration, HSV-2 could not affect HEp-2 cells. The present results indicated that Thymbra spicata L., with a minimum concentration value of 40 ?g/ml significantly showed antiviral effect against HSV-2 compared to the control group (p<0.001). This study shows that the essential oils of Thymbra spicata L. seems to have notable antiviral activity for HSV-2. We think that this antiviral effect of the essential oils of Thymbra spicata L may be attributed the abundant components in the structure such as Carvacrol, cymol, gama-terpinene and thymol. Our results confirmed that the essential oils of Thymbra spicata L. is a potential source of new and selective agent for the treatment of HSV-2 infection. However, further studies are needed to understand its antiviral mechanism and before making a recommendation.
Açıklama
S.C. PIELOREX S.A.; S.C. PRODSTEFANO S.R.L; S.C. TARO INDUSTRY S.A.; PRO COMOD SHOES SRL
4th International Conference on Advanced Materials and Systems, ICAMS 2012 -- 27 September 2012 through 29 September 2012 -- Bucharest -- 100613
4th International Conference on Advanced Materials and Systems, ICAMS 2012 -- 27 September 2012 through 29 September 2012 -- Bucharest -- 100613
Anahtar Kelimeler
Antiviral, GC-MS, HEp-2, HSV-2, Thymbra spicata L.
Kaynak
Proceedings of the 4th International Conference on Advanced Materials and Systems, ICAMS 2012
WoS Q Değeri
Scopus Q Değeri
N/A
