Molecular detection of Papillomavirus and immunohistochemical investigation of p53 gene expressions in bovine papillomas and fibropapillomas

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dc.authoridCoskun, Nuvit/0000-0001-7642-6460
dc.authoridAtaseven, V.Soydal/0000-0003-0802-0826
dc.authoridyilmaz, volkan/0000-0003-2752-5360
dc.authoridDogan, Firat/0000-0001-8656-3645
dc.authoridKURU, Mushap/0000-0003-4409-251X
dc.authoridNUHOGLU, HILMI/0000-0003-2530-2542
dc.authoridERMUTLU, CELAL SAHIN/0000-0002-8923-7682
dc.contributor.authorEmin, Karakurt
dc.contributor.authorNuvit, Coskun
dc.contributor.authorSerpil, Dag
dc.contributor.authorEnver, Beytut
dc.contributor.authorAtaseven, Veysel Soydal
dc.contributor.authorVolkan, Yilmaz
dc.contributor.authorFirat, Dogan
dc.date.accessioned2024-09-18T20:08:06Z
dc.date.available2024-09-18T20:08:06Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractPapilloma and fibropapilloma cases are quite common in cattle breeding, which cause economic losses due to decrease in the production of milk, meat, and also impair the quality of hide. In this study, we aimed to determine viral etiology and investigate p53 expression levels with immunohistochemical methods from a total of 30 cases. The study material was collected between 2013 and 2021 in Kars, Turkey. Paraffin embedded tissues were used for earlier cases in which the freshly specimens could not be provided. Cases were investigated for papillomavirus etiology with polymerase chain reaction (PCR) using FAP59/FAP64 and MY09/MY11 primer pairs. In 20 of the 30 cases papillomaviruses were identified, and 10 cases were identified as Bovine papillomavirus-1 (BPV-1), 1 case as BPV-2, 1 case as BPV-12, and 1 case as equus caballus papillomavirus 2 (EcPV-2) after sequence analysis. p53 immunostaining was also performed, and the cases were graded according to the positively stained cells. In conclusion BPV-12 was detected for the first time in our country, EcPV-2 was detected first time in cattle indicating cross species infection and p53 was staining most evident in BPV-1 and BPV-2 cases and BPV-12 and EcPV-2 was not stained.en_US
dc.description.sponsorshipScientific Research Projects Coordinatorship of Kafkas University [2019-TS-31]en_US
dc.description.sponsorshipThis study was funded by the Scientific Research Projects Coordinatorship of Kafkas University (2019-TS-31).en_US
dc.identifier.doi10.1007/s00203-022-02902-0
dc.identifier.issn0302-8933
dc.identifier.issn1432-072X
dc.identifier.issue5en_US
dc.identifier.pmid35461436en_US
dc.identifier.scopus2-s2.0-85128702074en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.1007/s00203-022-02902-0
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8631
dc.identifier.volume204en_US
dc.identifier.wosWOS:000786402000001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofArchives of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCattleen_US
dc.subjectImmunohistochemistryen_US
dc.subjectPapillomaen_US
dc.subjectPolymerase chain reactionen_US
dc.subjectp53en_US
dc.titleMolecular detection of Papillomavirus and immunohistochemical investigation of p53 gene expressions in bovine papillomas and fibropapillomasen_US
dc.typeArticleen_US

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