Yazar "Cantekin, Zafer" seçeneğine göre listele
Listeleniyor 1 - 20 / 30
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Antibacterial and antioxidant activities of liquidambar orientalis mill. various extracts against bacterial pathogens causing mastitis(2017) Ökmen, Gülten; Cantekin, Zafer; Alam, Mohammmad Intakhab; Türkcan, Onur; Ergün, YasarAntibiotic resistance is being constantly developed worldwide. Coagulase Negative Staphylococci (CNS) and Staphylococcus aureus are common causes of bovine subclinical mastitis. Bioactive compound of medicinal plants shows anti-microbial, antimutagenic and anti-oxidant effects. The anti-bacterial and anti-oxidant activities of Liquidambar orientalis (L. orientalis) extracts on subclinical mastitis causing bacteria in cows have not been reported to date. The aim of the present study was to examine antibacterial and anti-oxidant effects of L. orientalis leaf extracts on S. aureus and CNS isolated from cows with subclinical mastitis symptoms. In this study, 3.2 mg/mL minimum inhibitory concentration (MIC) of ethanol extracts of L. orientalis has shown to be a most potent anti-bacterial and anti-oxidant for all isolated bacterial species from mastitis cows. In this study, it was investigated anti-bacterial and anti-oxidant potentials of acetone, methanol and ethanol extracts of the L. orientalis. The acetone extract showed maximum inhibition zone against S. aureus numbered 17 (12 mm). In addition to antibacterial properties, anti-oxidant activity of L. orientalis extract was examined by ABTS [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)] free radical assay. Trolox was used as a positive control anti-oxidant. Ethanol extract exhibited a strong anti-oxidant activity like Trolox anti-oxidant which was effective at 2.58 mM concentration. Bioactive compounds of sweet gum may be useful to screening mastitis causing bacteria for clinical applications.Öğe Antibiotic susceptibility and molecular identification of antibiotic resistance genes of staphylococci isolated from bovine mastitis in Algeria(2015) Saidi, Radhwane; Cantekin, Zafer; Khelef, Djamel; Ergün, Yaşar; Solmaz, Hasan; Kaidi, RachidBu çalışma, Cezayir’de süt sığırlarındaki mastitis vakalarından izole edilen 21 stafilokok (10 Staphylococcus aureus ve 11 Koagulaz Negatif Stafilokok) suşunun Cezayir’de veteriner sahada sıklıkla kullanılan 12 antibiyotiğe karşı in vitro fenotipik ve genotipik direncinin belirlenmesi amacıyla yapıldı. Stafilokok izolatları disk difüzyon yöntemiyle test edildi. mecA, blaZ, aac-aph, ermA, ermC, tetK ve tetM direnç genleri ise PCR ile araştırıldı. Stafilokok izolatları penisilin (%95.23), oksasilin (%80.95), klindamisin (%80.95) ve eritromisine (%76.19) karşı yüksek oranda dirençli bulundu. 21 stafilokok izolatından 20 tanesinin metisilin dirençli ve çoklu antibiyotik direncine sahip olduğu belirlendi. Çoklu antibiyotik direncine sahip suşların bir çok antibiyotiğe karşı direnç paterni belirlendi antibiyotik I-XIII). Antibiyotik direnç genlerinin oranları ise mecA (%100), tetM (%100), blaZ (%42.85) şeklinde gerçekleşti. Bu çalışmada çoklu antibiyotik direncine de sahip metisilin dirençli stafilokokların yüksek prevalansı dikkat çekici idi. Bu çalışma Cezayir’de sığır mastitislerinden izole edilen stafilokoklarda metisilin direncini ortaya koyan ilk çalışmadır. Bu direncin ortaya konulması sürü bazında hastalığın tedavi, kontrol ve mastitis nedeniyle sürüden ayrılacak hayvanlar için karar verilmesine yardımcı olabilir.Öğe Antibiotic Susceptibility and Molecular Identification of Antibiotic Resistance Genes of Staphylococci Isolated from Bovine Mastitis in Algeria(Kafkas Univ, Veteriner Fakultesi Dergisi, 2015) Saidi, Radhwane; Cantekin, Zafer; Khelef, Djamel; Ergun, Yasar; Solmaz, Hasan; Kaidi, RachidThe study aimed to investigate the phenotypic and genotypic identification of in vitro antimicrobial susceptibility of 21 Staphylococci (10 Staphylococcus aureus and 11 Coagulase Negative Staphylococci) isolated from bovine mastitis to 12 antimicrobial drugs frequently using in veterinary medicine in Algeria. Isolates of staphylococci from bovine mastitis were tested for antibiotics with disc-diffusion method according to the National Committee for Clinical Laboratory Standards guidelines in the Mueller-Hinton agar, and resistant genes mecA, blaZ, aac-aph, ermA, ermC, tetK and tetM were detected by PCR. Staphylococci isolates showed high resistance to penicillin (95.23%), oxacillin (80.95%), clindamycine (80.95%), and erythromycin (76.19%) but, no resistance in all these strains was detected for gentamicin. Among 21 isolates of Staphylococci, 20 were found to be methicillin and multidrug resistant. Multidrug resistant strains exhibited several antibiogram patterns (antibiotic I to XIII). The distribution of antibiotic-resistant genes was mecA (100%) and tetM (100) followed by blaZ (42.85%). In the present study, the significant determination was the high prevalence of methicillin-resistant Staphylococci, which were resistant to multiple antibiotics. The finding of methicillin-resistant staphylococci from bovine mastitis is the first report in Algeria and revealed the status of resistant isolates in herd that might be helpful in treatment, controlling of resistant strains and for deciding culling of cows.Öğe Carriage Methicillin-Resistant Staphylococcus aureus in Poultry and Cattle in Northern Algeria(Hindawi Limited, 2018) Bounar-Kechih, Saliha; Taha Hamdi, Mossadak; Aggad, Hebib; Meguenni, Nacima; Cantekin, ZaferMultiresistant and especially Methicillin-Resistant Staphylococcus aureus (MRSA) poses a serious public health problem that requires their immediate identification and antibiotic resistance characteristics. In order to determine antibiotic resistance S. aureus poultry and bovine origin, 8840 samples were collected from slaughterhouses in the northern region of Algeria between years 2009 and 2014. 8375 samples were from an avian origin (1875 from laying hens and 6500 from broiler chickens) and the rest was from bovine origin. Bacteriological isolation and identification were made by classical culture method and antibiotic resistance patterns were determined by disc diffusion test. The prevalence of S. aureus was 42% in laying hens, 12% in broilers, and 55% in bovine samples. The prevalence of MRSA was 57%, 50%, and 31% in laying hens, broiler chickens, and bovine, respectively. While MRSA strains isolated from poultry showed cross-resistance to aminoglycosides, fluoroquinolones, macrolides, sulphonamides, and cyclins, those isolated from bovine also revealed similar multiresistance except for sulphonamide. This high percentage of methicillin resistance and multidrug resistance in S. aureus poultry and bovine origin may have importance for human health and curing of human infections. © 2018 Saliha Bounar-Kechih et al.Öğe Coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitis(Tubitak Scientific & Technological Research Council Turkey, 2007) Aslantas, Ozkan; Demir, Cemil; Turutoglu, Huelya; Cantekin, Zafer; Ergun, Yasar; Dogruer, GokhanThis study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the coagulase gene from 80 S. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.Öğe Coagulase gene polymorphism of staphylococcus aurreus isolated from subclinical bovine mastitis(2007) Aslantaş, Özkan; Demir, Cemil; Türütoğlu, Hülya; Cantekin, Zafer; Ergün, Yaşar; Doğruer, GökhanÖzet: Bu çalışma, üç farklı şehirden (Hatay, Gaziantep ve Burdur) toplanan mastitisli sığır sütlerinden izole edilen 80 Staphylococcus aureus susunun koagulaz gen polimorfizmini araştırmak amacıyla yapıldı. Seksen S. aureus susu 5 farklı polymerase chain reaction (PCR) amplikonu oluştururken, 79 izolat 1 amplikon ve 1 izolat 2 amplikon oluşturdu. İzolatlar, koagulaz geninin restriction fragment length polymorphism (RFLP) analizi ile 9 genotipe ayrıldı. İki yaygın genotip izolatların % 73,8'ini oluşturdu. Sonuçlar, çalışılan bölgelerde farklı genotiplerin olduğunu ve bunlardan sadece bir veya iki genotipin dominant olduğunu göstermektedirÖğe Comparison of PCR and culture methods for diagnosis of subclinical mastitis in dairy cattle(2015) Cantekin, Zafer; Ergün, Yaşar; Doğruer, Gökhan; Sarıbay, Mustafa Kemal; Solmaz, HasanSığır mastitisleri süt sığırcılığı endüstrisinde ekonomik kayıpların en önemli nedenidir. Mastitiste etkeninin hızlı ve kesin tanısı hastalığın tedavisi ve kontrolü açısından çok önemlidir. Bu çalışmanın amacı subklinik sığır mastitislerinde etkenin tanısı için kültür ve polimeraz zincir reaksiyonu tekniklerinin karşılaştırılmasıdır. Bu amaçla, 540 ineğin California Mastitis Test ile muayenesinden sonra, pozitif bulunan 79 adet süt örneği, klasik kültür metodu ve direkt sütten yapılan polimeraz zincir reaksiyonu ile analiz edildi. Kırk üç örnekte her iki yöntemde de mikroorganizma belirlenirken, sadece 7 örnekte iki teknik arasından fark görüldü. Bu 7 örnekte kültür yöntemiyle sadece Koagülaz Negatif Stafilok belirlenirken, polimeraz zincir reaksiyonu ile Koagülaz Negatif Stafilok dışında S. dysgalactiae yönünden de pozitif bulundu. Bu çalışmanın sonuçları polimeraz zincir reaksiyonunun kültür yönteminden daha duyarlı olduğunu ve direkt olarak süt numunesinden hedef etkenin bir kaç saat içinde saptanabileceğini gösterdi. Hızlı ve güvenilir moleküler tekniklerin kullanımı mastitiste sürüden çıkarma veya tedavi etme konusunda hızlı karar verilebilmesinde yardımcı olabilir.Öğe Cystitis and Bilateral Pyonephrosis in a Mixed Breed Goat(Univ Agriculture, Fac Veterinary Science, 2018) Kose, Serkan Irfan; Kanat, Ozgur; Cantekin, Zafer; Ozturk, Aliye Sagkan; Erturk, AlperPyonephrosis defined as suppurative destruction of the parenchyma of the kidney and rarely reported in domestic animals The aim of this case report was to evaluate pyonephrosis in the goat by clinical, laboratory and ultrasonographic examinations. A 4-years-old mixed breed doe goat in 45 kg body weight, having complaints with abdominal distension, dysuria, strangury, abdominal pain, groaning, and lethargy, was used. General physical and laboratory examinations, ultrasonographic imaging, and microbiological analysis were performed. Goat was euthanized by the owner request because of bad clinical situation and prognosis. Pyonephrosis and cystitis were determined in this mixed breed goat in the light of clinical, laboratory, ultrasonographic imaging, and especially post-mortem examinations. In conclusion, the results of this case report will provide important insights into pyonephrosis less frequently seen in goats in the clinics. (C) 2018 PVJ. All rights reservedÖğe Detection of virulence properties Escherichia coli originated from poultry by phenotypic and molecular methods(2015) Cantekin, Zafer; İzgür, MüjganKanatlı Orijinli Escherichia coli suşlarının virülens özelliklerinin fenotipik ve moleküler metotlarla belirlenmesi*Bu çalışmada, kolibasillozisli kanatlı hayvanlardan izole edilen 200 adet Escherichia (E.) coli suşunda, özellikle ekstra-intestinal infeksiyonlar için önemli olan virülens faktörlerinden serum direnci, aerobaktin demir elde etme sistemleri, çeşitli adezinler (Tip 1 fimbrialar, P fimbrialar, S fimbrialar ve afimbrial adezinler), hemoliz ve sitotoksik nekrotizan faktör 1 gibi virülens özellikleri fenotipik yöntemlerle ve moleküler yöntemlerle araştırıldı. Çalışmada, suşlar koyun kanlı agar kullanılarak yapılan testle hemoliz üretimi ve moleküler olarak hemoliz özelliğini kodlayan gen yönünden negatif bulundu. Suşların tamamının demir kısıtlayıcı besiyerinde ürediği ve bu suşların %88'inin aerobaktin özelliğini kodlayan İucD genine sahip oldukları saptandı. Vero hücre kültürleri kullanılarak yapılan toksin analizlerinde suşların %40'ında ısıya duyarlı toksin belirlenirken suşların hiç birinde fenotipik ve moleküler olarak sitotoksik nekrotizan faktör 1 belirlenmedi. İnsan O grubu ve değişik hayvanlara ait eritrositler kullanılarak yapılan hemaglutinasyon testi ile suşların %90'ında hemaglutinasyon aktivitesi saptandı ve bunların %26'sı mannoz resistans hemaglutinasyon özelliğinde olduğu belirlendi. Tip 1 fimbriaları kodlayan FimH geni suşların %88'inde, P fimbriaları kodlayan PapEF geni %26'sında bulundu ve bu izolatların %14'ü aynı zamanda PapC genini de bulundurduğu saptandı. Ancak, suşlarda S fimbrialar ve afimbrial adezinleri kodlayan Sfa ve Afa genleri belirlenemedi. Değişik hayvanlara ait serumlarla yapılan testte suşların tamamında serum direnci fenotipik olarak saptandı ve serum direncinden sorumlu TraT geni suşların %90'ında PCR ile belirlendi. Bu çalışmada elde edilen sonuçlara göre, kanatlı orijinli E. coli suşlarında en önemli virülens özelliklerinin aerobaktin demir elde etme sistemleri, fimbrial adezinler ve serum direnci olduğu belirlenmiştirÖğe Development of pmp Gene-Specific PCR Assay with A Host Specific Internal Control for Chlamydophila felis(Chulalongkorn Univ, 2014) Cantekin, Zafer; Solmaz, Hasan; Altug, Nuri; Ozmen, Gamze OzgeChlamydophila felis is the primary bacterial agent of conjunctivitis and upper respiratory disease in cats. Transmission of the disease requires close contact between cats. Polymerase chain reaction is a useful tool for detection of this organism. The aim of this study was to develop a polymerase chain reaction assay with an internal amplification control for the detection of C. felis. Primer pairs were designed specifically for polymorphic membrane protein gene of C. felis and cytochrome b gene of cat, and their specificity and sensitivity were examined. Primers specific for both genes were then multiplexed. In the simplex polymerase chain reaction analyses with 10-fold dilutions, C. felis DNA was detected with designed primers for polymorphic membrane protein genes up to 1.6 pg/mu l and cat DNA was demonstrated in all samples in the polymerase chain reaction. Moreover, in the multiplex polymerase chain reaction, C. felis DNA and cat DNA were detected together. These designed primers specific for C. felis might have potential for research on infections and shedding of this organism in cats as the internal control host specific primers might have potential for using internal control for detection of different microorganisms in cats.Öğe Development of pmp gene-specific PCR assay with a host specific internal control for Chlamydophila felis(Chulalongkorn University Printing House, 2014) Cantekin, Zafer; Solmaz, Hasan; Altug, Nuri; Ozmen, Gamze OzgeChlamydophila felis is the primary bacterial agent of conjunctivitis and upper respiratory disease in cats. Transmission of the disease requires close contact between cats. Polymerase chain reaction is a useful tool for detection of this organism. The aim of this study was to develop a polymerase chain reaction assay with an internal amplification control for the detection of C. felis. Primer pairs were designed specifically for polymorphic membrane protein gene of C. felis and cytochrome b gene of cat, and their specificity and sensitivity were examined. Primers specific for both genes were then multiplexed. In the simplex polymerase chain reaction analyses with 10-fold dilutions, C. felis DNA was detected with designed primers for polymorphic membrane protein genes up to 1.6 pg/?l and cat DNA was demonstrated in all samples in the polymerase chain reaction. Moreover, in the multiplex polymerase chain reaction, C. felis DNA and cat DNA were detected together. These designed primers specific for C. felis might have potential for research on infections and shedding of this organism in cats as the internal control host specific primers might have potential for using internal control for detection of different microorganisms in cats.Öğe A Duplex PCR for detection of S. aureus and Staphylococcus spp. from culture and bovine milk samples(2014) Cantekin, Zafer; Saidi, Radhwane; Solmaz, Hasan; Ergün, YaşarMastitis süt sığırcılığı endüstrisinin en fazla ekonomik kayba neden olan problemidir. Stafilokok türleri en sık izole edilen etkenler olup, Staphylococcus aureus ise süt sığırlarında en önemli kontagiyöz etkendir. Bu çalışmanın amacı stafilokok türleri ve S. aureus’un kültür ve süt örneklerinden teşhisi için ikili bir PZR tekniğinin geliştirilmesidir. Çalışmada pozitif kontrol olarak, S. aureus (ATCC 25923) ve S. epidermidis (ATCC 12228) DNA’sı kullanıldı. Geliştirilen tekniğin denenmesi amacıyla Veteriner Hekimlerce laboratuvarımıza gönderilen örneklerden bir adet koagulaz negatif stafilokok bir adet de S. aureus pozitif süt örneği klinik örnek olarak kullanıldı. Çalışmada sütten ve kültür örneklerinden stafilokok ve S. aureus’un teşhisi ve ayrımı için ikili ve hızlı bir PZR tekniği geliştirildi. Geliştirilen bu tekniğin stafilokok türleri ve S. aureus’un bir kaç saat içinde hem süt hem de kültür örneklerinden tanısı ve ayrımı için alternatif, güvenilir ve hızlı bir tanı yöntemi olarak özellikle sonuçların çiftlik bazında kullanılmak üzere klinik ve subklinik olgularda ineğin tedavi edilmesi ya da kesime gönderilmesine karar verilmesi amacıyla kullanılabileceği ortaya konuldu.Öğe The Effect of Cefovecin Sodium in Shelter Dogs with Bacterial Lower Respiratory Disease(Inst Tecnologia Parana, 2023) Kose, Serkan Irfan; Ozer, Burcin; Gonenci, Ramazan; Cantekin, ZaferThis study evaluated the clinical and bacteriological efficacy of cefovecin sodium in shelter dogs with bacterial lower respiratory disease. All dogs (n = 32) with lower respiratory disease were divided into two treatment groups: the cefovecin (n = 16) and the ceftriaxone (n = 16) groups. On the first five days and the 8th day of treatment, and after treatment (15th day), the examination of all dogs was performed. Blood analysis and thoracic radiographic imaging were done. In bronchoalveolar lavage fluids, in the cefovecin group, Bordetella bronchiseptica (n=13), Staphylococcus spp. (n=9), Streptococcus spp. (n=7), Klebsiella pneumonia (n=1); in the ceftriaxone group; B. bronchiseptica (n=5), Escherichia coli (n=5), Pasteurella canis (n=4), Streptococcus spp. (n=3), Staphylococcus aureus (n=1), Pasteurella aerogenes (n=1) and Klebsiella oxytoca (n=1) were isolated and identified. Cefovecin and ceftriaxone sodium treatment protocols had anti-bacterial efficacies of 68.75% and 100%, respectively. In light of the study results, it is concluded that although cefovecin sodium looks to be an antibacterial drug that may be used to treat bacterial lower respiratory tract infections in shelter dogs due to its ease of use, cefovecin and other cephalosporins should not be used empirically as they may contribute to bacterial resistance.Öğe Effect of different plant extracts on microbial population in the frozen African Catfish (Clarias gariepinus) semen(C M B Assoc, 2023) Secer, Faik Sertel; Yavas, Ilker; Cantekin, Zafer; Bozkurt, Yusuf; Yavas, Tugba Korkmaz; Atanasoff, AlexanderFish sperm cryopreservation has been attempted on roughly freshwater and marine species since 1953. This study sought to assess the potential of various plant extracts to function as natural antimicrobial agents in the frozen semen of African catfish (Clarias gariepinus). Diluted sperm was packaged in 0.25ml straws and left for 10min equilibration at 4 degrees C. Following equilibration, the straws were exposed to liquid nitrogen vapor for 10 min and plunged into the liquid nitrogen (-196 degrees C) and then thawed in a water bath at 35 degrees C for 20s. Sperm samples were put into sterile 1.5 ml tubes immediately after thawing and the microbial count was detected with classical microbiological culture method. In the results of microbiological analyses, these tree plant extracts especially Echinacea purpurea were found highly effective for decreasing bacterial contamination levels of African catfish (C. gariepinus) semen. These plant extracts may have the potential for antibacterial effect, and they can be useful for the dilution of semen.Öğe Effects of inactivated brewer's yeast (saccharomyces cereviciae) on egg production, serum antibody titres and cholesterol levels in laying hens(2013) Sacakli, Pinar; Ergun, Ahmet; Koksal, Bekir Hakan; Ozsoy, Bulent; Cantekin, ZaferThe aim of this study was to investigate the effects of dietary supplementation with inactivated brewer's yeast (Saccharomyces cereviciae) produced from waste of beer industry, on egg production, feed efficiency, serum antibody titers and cholesterol levels in laying hens. A total of 320, twenty two wks old laying hens (Lohmann Brown) were randomly divided into 4 equal groups (each of them 80 hens) with 4 replicates according to the diet regimen; birds were supplemented for 16 wks with 1, 3, 5% Saccharomyces cereviciae whereas in the control group, layers were not supplemented. All diets were formulated to meet or exceed the National Research Council (NRC, 1994) recommended layer requirements for all nutrients and study was carried out for 16 wks. Egg production, feed intakes and feed efficiency were determined biweekly whereas serum antibody titers and cholesterol levels were explored on wks 2 and 16 in 15 birds from each group. At the end of the study, there were no significant effects of yeast supplementation on egg production, feed intake and feed conversion ratio in laying hens whereas there were significant differences (P<0.05) between control and other treatment groups about feed conversion ratio parameters at 28-29th wks of study. Similar to serum antibody titers, cholesterol levels of laying hens also were not altered by yeast supplementation. As a conclusion, Saccharomyces cereviciae had no beneficial effect on production parameters of hens fed with optimal diets and reared under proper management conditions.Öğe Effects of Kefir on Blood Parameters and Intestinal Microflora in Rats: An Experimental Study(Kafkas Univ, Veteriner Fakultesi Dergisi, 2021) Ozsoy, Bulent; Cantekin, Zafer; Yalcin, Sakine; Bayraktar, Hamdullah SuphiA probiotic product of kefir is widely consumed by human beings. The purpose of this research was to investigate the effects of kefir on blood parameters and intestinal flora in rats. A total of 24 female rats were used in this study. During 35 days of experimental period, rats were fed with a commercial diet and water was provided ad libitum. Kefir was given at the levels of 10 mL/kg, 20 mL/kg and 30 mUkg with oral gavage to the first, second and third treatment groups, respectively. Kefir was not given to the control group. The number of yeast was found to be 1.65x10(8) and the number of lactobacilli was found to be 4x10(8) in kefir. At the end of the experiment, blood samples were taken from all rats. Blood plasma parameters and were investigated. The intestinal microflora was investigated by classical colony counting method. No differences were observed among the groups in total protein, albumin, uric acids, SGPT, SGOT, alkaline phosphatase and phosphorus in blood plasma. The plasma triglyceride and cholesterol levels in the second and third groups were lower than control group (P<0.05). No differences were observed in the intestinal pH levels among groups. Although total bacteria number of intestinal microflora was not different in groups, the number of Enterobactericeae and coliform bacteria in the third group was lower than the other groups (P<0.001). The number of Lactobacilli and the yeast level in the intestinal contents were increased by the usage of kefir (P<0.001). It was concluded that positive effects of the kefir were observed in intestinal microflora with increasing the number of beneficial bacteria and decreasing harmful bacteria and therefore kefir has a positive effect on the health of the animals.Öğe Emerging of antimicrobial resistance in staphylococci isolated from clinical and food samples in Algeria(BioMed Central Ltd., 2018) Achek, Rachid; Hotzel, Helmut; Cantekin, Zafer; Nabi, Ibrahim; Hamdi, Taha Mossadak; Neubauer, Heinrich; El-Adawy, HosnyObjective: The antimicrobial resistance of staphylococci rose worldwide. In total, 96 Staphylococcus isolates from food and clinical samples were collected from two provinces in Algeria. The antimicrobial susceptibility testing was performed and resistance-associated genes were detected. Results: Fifty-one strains were isolated from food samples and differentiated into 33 Staphylococcus aureus and 18 coagulase-negative staphylococci. Forty-five staphylococci were collected from hospital and community-acquired infection cases. All S. aureus isolated from food were resistant to penicillin and 45.5% were resistant to tetracycline. The resistance rates of 45 clinical Staphylococcus isolates were 86.7%, 48.9%, 37.8% and 20.0% to penicillin, tetracycline, erythromycin and kanamycin, respectively. Nine isolates were confirmed as MRSA from food and clinical isolates. One S. aureus originated from food was confirmed as vancomycin-resistant. Multidrug-resistance was observed among 25.5% and 53.3% of food and clinical staphylococci, respectively. The tetM/K, blaZ, aacA-aphD, ermC and mecA genes were detected in food and clinical isolates. ermA gene was not found. This study provided insight into the status of antimicrobial resistance of staphylococci isolated from food and clinical samples in Algeria. Further investigations and surveillance programmes are mandatory. © 2018 The Author(s).Öğe Güvercin (Columbia domestica) dışkılarında chlamydia psittaci ompa geninin araştırılması ve genotiplendirilmesi(Ankara Mikrobiyoloji Derneği, 2020) Altıntaş, Özlem; Ünal, Nilgün; Karagöz, Alper; Cantekin, ZaferKanatlı klamidiyozisi evcil ve yabani kanatlılarda görülebilen, oldukça bulaşıcı ve sistemik bir hastalıktır. Hastalık etkeni Chlamydia psittaci, Chlamydiaceae familyasında, gram-negatif ve sadece hücre içerisinde yaşayabilen bir bakteridir. Etken insanlara, enfekte hayvanla temas ve dışkı aracılığıyla bulaşabileceği gibi etkeni içeren dışkı tozlarının solunmasıyla da bulaşabilir. Hastalık zoonoz bir karakter taşıdığı için halk sağlığı yönüyle de önemlidir. C.psittaci etkeninin hücre duvarı proteinlerine (OMP) karşı monoklonal antikorların kullanımıyla kanatlılarda altı (A-F), memelilerde iki (WC ve M56) serotip belirlenmiştir. Serotiplerde konak özgüllüğü vardır. Moleküler genotiplendirmede hücre duvarı proteinlerini kodlayan “major outer membrane protein/MOMP” ompA geni kullanılmaktadır. Bu çalışmada, Ankara’da on farklı ilçede bulunan aile işletmeciliği tarzında yetiştirilen, evcil güvercin dışkılarında C.psittaci ompA geni varlığının kış ve yaz mevsimlerinde araştırılması ve ompA geninin dizi analizi ile genotiplendirilmesi amaçlanmıştır. Çalışma kapsamında Ankara ilinde 10 farklı ilçede (Beypazarı, Haymana, Kızılcahamam, Çubuk, Pursaklar, Bala, Çankaya, Polatlı, Gölbaşı ve Merkez) bulunan kuşhanelerden iki farklı mevsimde 100 güvercin dışkı örneği toplanmıştır. Toplanan dışkı örneklerinden DNA eldesi klasik yöntemlerle yapılmıştır. Dışkıdan elde edilen DNA örneklerinde etkenin varlığı ompA geninin polimeraz zincir reaksiyonu (PCR) analizi ile araştırılmıştır. PCR ile çoğaltılan hedef DNA ürünlerinden çalışmada kullanılan primerler ile ompA geninin çift yönlü dizi analizi yapılmıştır. Dizi analizi sonuçları uluslararası veri tabanı ile karşılaştırılarak serotiplendirme/ genotiplendirme yapılmıştır. Çalışmada 100 güvercin dışkı örneğinden altı örnekte C.psittaci ompA geni tespit edilmiştir. Bu pozitif örneklerin ikisi Bala (bir kış ve bir yaz dönemi numunesi), ikisi Haymana (bir kış, bir yaz dönemi numunesi), ikisi Gölbaşı (bir kış, bir yaz dönemi numunesi) ilçelerinden olmak üzere aynı kuşhanelerden farklı mevsimlerde izole edilmiştir. Bu çalışma ile güvercin dışkılarında C.psittaci’nin varlığı ile mevsim arasında bir fark belirlenmemiştir. Ayrıca, bu çalışmada pozitif örneklerde ompA geni dizi analiz sonuçlarının Dünya veri bankası ile karşılaştırıldığında bütün izolatların %100 oranında genotip B, %99 oranında da genotip E ile uyumlu olduğu görülmüştür. Türkiye’de, ompA geninin dizi analizi ile evcil güvercin kökenli C.psittaci genotipi ilk kez bu çalışmada belirlenmiştir. C.psittaci’nin zoonoz bir bakteri olması ve evcil güvercinlerde düşük oranda da olsa bulunması halk sağlığı açısından önemlidir.Öğe Hatay ilindeki aile tipi süt sığırcılığı işletmelerinde subklinik mastitislerin epidemiyolojisi(2004) Ergün, Yaşar; Aslantaş, Özkan; Doğruer, Gökhan; Cantekin, ZaferBu çalışma, Hatay ilinde işletme başına 3-10 baş inek yetiştirilen aile.tipi süt sığırcılığı işletmelerinde yürütüldü. Bu amaçla, 11 ilçeye bağlı farklı yerleşim yerlerinden alınan toplam 160 süt ineği Califoma Mastitis Test'i (CMT) ile muayene edildi. CMT pozitif 115 (% 71.9) inekten alınan 262 (% 40.9) adet süt örneği mikrobiyolojik olarak incelendi. İzole ve identifiye edilen mikroorganizmaların 12 farklı antibiyotiğe karşı duyarlılıkları belirlendi. Mikrobiyolojik olarak incelenen 262 adet süt örneğinin 200'ünden (% 76.3) aerobik mikroorganizma izole ve identifiye edildi. Bu etkenlerin 98'i (% 42.4) Koagulaz Negatif Staph-ylococcus (KNS), 58'i (% 25.1) S. aureus, 26'sı (% 11.2) S. uberis, 15'i (% 6.5) S.agalactiae, 8'i (% 3.5) S.dysgalactiae, 6'sı (% 2.6) Micrococcus spp, 5'i (% 2.2) Bacillus spp., 5'i (% 2.2) £ coli, 4'ü (% 1.7) Candida spp., 4'ü (% 1.7) P. auriginosa ve 2'si (% 0.9) S. feacalis olarak tespit edildi, izole ve identifiye edilen mikroorganizmalara karşı en etkili antibiyotiklerin Da-nofloksasin, Enrofloksasin, Sulbaktam-Ampisilin, Amoksisilin-Klavulonik Asit ve Sefaperazon olduğu belirlendi. Sonuç olarak, bölgedeki aile tipi sütçü işletmelerde subklinik mastitis prevalansının yüksek olduğu ve sahada yaygın olarak kullanılan bazı antibiyotiklere karşı direnç geliştiği belirtendi.Öğe Investigation into toxin and slime genes in staphylococci isolated from goat milk and goat cheese in southern Turkey(Chulalongkorn Univ, 2018) Onen, Sevda Pehlivanlar; Cantekin, Zafer; Mebkhout, Faiza; Aygun, Osman; Ergun, YasarThe aim of this study was to investigate the presence of Staphylococcus aureus and coagulase-negative staphylococci (CNS) isolated from goat milk and cheese, as well as their toxin genes and slime genes. S. aureus and CNS isolates were subjected to polymerase chain reaction (PCR) analyses to determine the prevalence of enterotoxin (sea, seb, sec, sed, see), toxic shock syndrome (TSS) toxin (tst), exfoliative toxin (eta and etb) and slime genes (icaA and icaD). sec, sed and tst genes together were detected in 4 (33.3%), sec + tst genes in 2 (16.7%) and tst gene in 1 (8.3%) of the 12 S. aureus isolates. However, see gene was detected in 6 (9.7%), sed gene and tst gene were detected in 1 (1.6%) and 7 (11.3%) of the 62 CNS isolates, respectively. No sea, seb, see, eta or etb genes were detected in the S. aureus isolates, whereas no sea, seb, sec, eta or etb genes were detected in the CNS isolates. At least one ica gene was present in all S. aureus isolates and in 36 of the 62 CNS isolates. The presence of icaA and icaD genes in the S. aureus isolates was clearly higher than those in the CNS isolates. In conclusion, toxin and slime genes were detected in the S. aureus and CNS isolates from goat milk and goat cheese. The potential risk of enterotoxigenic CNS should not be ignored as well as S. aureus in food safety and public health.
